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Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated D1-W3, was isolated from tidal flat sediment of the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain D1-W3 grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic analyses based on 16S rRNA gene sequences revealed that strain D1-W3 fell within the clade comprising species of the genus , clustering with the type strains of , and with which it exhibited the highest 16S rRNA gene sequence similarity values (98.1–98.2 %). The 16S rRNA gene sequence similarity values between strain D1-W3 and the type strains of other species of the genus were in the range 93.5–96.5 %. The DNA G+C content of strain D1-W3 was 58.1 mol% and the mean DNA–DNA hybridization values with KCTC 12722, LMG 22534 and DSW-18 were 13–25 %. Strain D1-W3 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, showed that strain D1-W3 could be differentiated from other species of the genus . On the basis of the data presented, strain D1-W3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D1-W3 ( = KCTC 32383 = CECT 8284).

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2013-11-01
2019-10-17
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