1887

Abstract

Two Gram-positive, catalase-negative bacterial strains were isolated from the cloaca of common kingfishers (). Repetitive sequence-based PCR fingerprinting using the (GTG) primer grouped these isolates into a single cluster separated from all known enterococcal species. The two strains revealed identical 16S rRNA gene sequences placing them within the genus with LMG 16607 as the closest relative (97.14 % similarity). Further taxonomic investigation using sequencing of the genes for the superoxide dismutase (), phenylalanyl-tRNA synthase alpha subunit () and the RNA polymerase alpha subunit () as well as application of whole-cell protein fingerprinting, automated ribotyping and extensive phenotyping confirmed that both strains belong to the same species. Based on data from this polyphasic study, these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L34 ( = CCM 8433 = LMG 27164).

Funding
This study was supported by the:
  • Ministry of Education, Youth and Sports of the Czech Republic (Award CZ.1.07/2.3.00/20.0183)
  • European Regional Development Fund (Award CZ.1.05/1.1.00/02.0068)
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2013-08-01
2024-12-13
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