1887

Abstract

A Gram-stain-negative, aerobic, catalase- and oxidase-positive, non-flagellated, rod-shaped bacterium, designated strain P-50-3, was isolated from seawater of the Pacific. The strain grew at 10–40 °C (optimum at 30 °C) and with 0–12 % (w/v, optimum 2 %) NaCl. It reduced nitrate to nitrite but did not hydrolyse gelatin, starch or Tween 80. Analysis of 16S rRNA gene sequences showed that strain P-50-3 clustered tightly with the genus and shared the highest 16S rRNA gene sequence similarity (94.3 %) with the type strain of . The major respiratory quinone was Q-10 and the major cellular fatty acids were Cω7, C, 11-methyl Cω7 and C. Polar lipids included phosphatidylglycerol (PG), phosphatidylcholine (PC), two unidentified aminolipids and an unidentified lipid. The genomic DNA G+C content of strain P-50-3 was 69.0 mol%. On the basis of the data obtained in this polyphasic study, strain P-50-3 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain of is P-50-3 ( = KACC 16527 = CGMCC 1.11030). An emended description of the genus Lim 2008 is also proposed.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 41276149, 31025001, 31070061, 31170055, 81271896, 41106161, 31290231 and 41176130)
  • , Hi-Tech Research and Development Program of China , (Award 2012AA092103 and 2011AA090703)
  • , Special Fund of China for Marine Scientific Research in the Public Interest , (Award 201005032-6)
  • , China Ocean Mineral Resources R & D Association (COMRA) Special Foundation , (Award DY125-15-R-03 and DY125-15-T-05)
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2013-10-01
2020-07-10
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