1887

Abstract

A yellow-coloured bacterial strain, designated HB2, isolated from stratum water was investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a member of the genus , its three closest neighbours being BCRC 17731 (97.5 % similarity), JCM 12488 (97.3 %) and DSM 19680 (96.9 %). Strain HB2 could clearly be distinguished from these type strains based on phylogenetic analysis, DNA–DNA hybridization, fatty acid composition and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strain HB2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HB2 ( = DSM 26429 = CICC 11005s).

Funding
This study was supported by the:
  • Huabei Oilfield Company (Award 2012-HB-z231)
  • China National Research Institute of Food and Fermentation Industries (Award 2012kJFZ-BS-01)
  • National Science and Technology Support Plans of China (Award 2012BAK17B11)
  • International Science and Technology Cooperation Projects of Beijing (Award Z111105054611011)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049718-0
2013-09-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3352.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049718-0&mimeType=html&fmt=ahah

References

  1. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N. ( 2008 ). Luteimonas marina sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58, 29042908. [View Article] [PubMed]
    [Google Scholar]
  2. Chou J.-H., Cho N.-T., Arun A. B., Young C.-C., Chen W.-M. ( 2008 ). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. . Int J Syst Evol Microbiol 58, 20512055. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267288. Edited by Goodfellow M., O’Donnell A. G. . London:: Academic Press;.
    [Google Scholar]
  4. Collins M. D., Jones D. ( 1981 ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316354.[PubMed]
    [Google Scholar]
  5. Eck R. V., Dayhoff M. O. ( 1966 ). Atlas of Protein Sequence and Structure. Silver Springs, MD:: National Biomedical Research Foundation;.
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridizationin in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  11. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  12. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematic, pp. 115175. Edited by Stackerandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  14. Roh S. W., Kim K.-H., Nam Y.-D., Chang H.-W., Kim M.-S., Yoon J.-H., Oh H.-M., Bae J.-W. ( 2008 ). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. . J Microbiol 46, 525529. [View Article] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  19. Yokota A., Akagawa-Matsushita M., Hiraishi A., Katayama Y., Urakami T., Yamasato K. ( 1992 ). Distribution of quinone systems in microorganisms: Gram-negative Eubacteria. . Bull Jpn Fed Cult Coll 8, 136171.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049718-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049718-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error