1887

Abstract

A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (B1315) was isolated from the placenta of a sheep with abortion. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the group with 94.5–94.8 %, 94.3–96.1 %, 95.0–95.1 %, and 95.9–96.1 % sequence similarities to type strains of species of the four genera, respectively. Phylogenetic trees indicated a close relationship to the type strains of and (95.9 and 96.1 % sequence similarity, respectively). Chemotaxonomic data confirmed the allocation of strain B1315 to the family (quinone system: ubiquinone Q-10 and major fatty acids: Cω7 and C cyclo ω8). The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and the unidentified but unique phospholipid PL7. The polyamine pattern of strain B1315 contained the major polyamines spermidine and putrescine. On the basis of the 16S rRNA gene and sequence phylogeny and chemotaxonomic data strain B1315 was clearly different from the genera , , and . On the basis of these data we propose the novel genus gen. nov. with the type species sp. nov. with the type strain B1315 ( = CCM 8460 = LMG 27356) The taxonomic allocation of , which grouped inconsistently together with strain B1315 on the basis of 16S rRNA gene sequence data, but shows the chemotaxonomic features of the genus , remains to be clarified.

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2013-10-01
2019-10-17
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J.. ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47:, 39–52. [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F.. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef][PubMed]
    [Google Scholar]
  3. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. ( 2005;). phylip (phylogeny inference package) version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Jones D. T., Taylor W. R., Thornton J. M.. ( 1992;). The rapid generation of mutation data matrices from protein sequences. . Comput Appl Biosci 8:, 275–282.[PubMed]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of the protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Steiof M., Dott W.. ( 1991;). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21:, 227–251. [CrossRef]
    [Google Scholar]
  13. Kämpfer P., Rosselló-Mora R., Scholz H. C., Welinder-Olsson C., Falsen E., Busse H. J.. ( 2006;). Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp. nov. and Pseudochrobactrum saccharolyticum sp. nov.. Int J Syst Evol Microbiol 56:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  14. Kämpfer P., Scholz H., Huber B., Thummes K., Busse H.-J., Maas E. W., Falsen E.. ( 2007;). Description of Pseudochrobactrum kiredjianiae sp. nov.. Int J Syst Evol Microbiol 57:, 755–760. [CrossRef][PubMed]
    [Google Scholar]
  15. Kämpfer P., Huber B., Lodders N., Warfolomeow I., Busse H.-J., Scholz H. C.. ( 2009;). Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid. . Int J Syst Evol Microbiol 59:, 2464–2467. [CrossRef][PubMed]
    [Google Scholar]
  16. Kämpfer P., Martin E., Lodders N., Jäckel U., Huber B. E., Schumann P., Langer S., Busse H. J., Scholz H.. ( 2010;). Paenochrobactrum gallinarii gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov.. Int J Syst Evol Microbiol 60:, 1493–1498. [CrossRef][PubMed]
    [Google Scholar]
  17. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  18. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). ARB: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  20. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  21. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V.. ( 2008;). Pseudochrobactrum glaciei sp. nov., isolated from sea ice collected from Peter the Great Bay of the Sea of Japan. . Int J Syst Evol Microbiol 58:, 2454–2458. [CrossRef][PubMed]
    [Google Scholar]
  22. Scholz H. C., Tomaso H., Al Dahouk S., Witte A., Schloter M., Kämpfer P., Falsen E., Neubauer H.. ( 2006;). Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. . FEMS Microbiol Lett 257:, 7–16. [CrossRef][PubMed]
    [Google Scholar]
  23. Scholz H. C., Al Dahouk S., Tomaso H., Neubauer H., Witte A., Schloter M., Kämpfer P., Falsen E., Pfeffer M., Engel M.. ( 2008;). Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum–Brucella group by recA and 16S rRNA gene-based comparative sequence analysis. . Syst Appl Microbiol 31:, 1–16. [CrossRef][PubMed]
    [Google Scholar]
  24. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Tindall B. J.. ( 1990;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  27. Ventosa A., Marquez M. C., Kocur M., Tindall B. J.. ( 1993;). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. . Int J Syst Bacteriol 43:, 245–248. [CrossRef][PubMed]
    [Google Scholar]
  28. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K.-H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31:, 241–250. [CrossRef][PubMed]
    [Google Scholar]
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