1887

Abstract

A Gram-negative, rod-shaped bacterium was isolated from the skin of the medical leech and studied for its taxonomic allocation. 16S rRNA gene sequence similarities to other strains showed that the strain was closely related to species of the genus was shown to be the most closely related species (96.8 %), followed by (96.6 %) and (96.2 %). All other species of the genus showed 16S rRNA gene sequence similarities <96 %. Chemotaxonomic data for strain E94 (major ubiquinone: Q-10; major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylserine, unknown phospholipids, aminolipids and aminophospholipids; and major fatty acids: Cω7, Cω8 cylco, C and C) supported the affiliation of the isolate to the genus . Phenotypic differentiation of strain E94 from all species of the genus was possible using different physiological characters. Strain E94 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain E94 ( = LMG 26925 = CIP 110381 = CCM 8401).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049619-0
2013-09-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3209.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049619-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 48014805. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459470. [View Article]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  5. Felsenstein, J. (2005). phylip (phylogeny inference package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [View Article] [PubMed]
    [Google Scholar]
  8. Im W.-T., Yokota A., Kim M.-K., Lee S.-T. ( 2004 ). Kaistia adipata gen. nov., sp. nov., a novel α-proteobacterium. . J Gen Appl Microbiol 50, 249254. [View Article] [PubMed]
    [Google Scholar]
  9. Jin L., Kim K. K., Baek S.-H., Lee S.-T. ( 2011 ). Kaistia geumhonensis sp. nov. and Kaistia dalseonensis sp. nov., two members of the class Alphaproteobacteria . . Int J Syst Evol Microbiol 61, 25772581. [View Article] [PubMed]
    [Google Scholar]
  10. Jin L., Kim K. K., Lee H.-G., Ahn C.-Y., Oh H.-M. ( 2012 ). Kaistia defluvii sp. nov., isolated from river sediment. . Int J Syst Evol Microbiol 62, 28782882. [View Article] [PubMed]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of the protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  13. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227251. [View Article]
    [Google Scholar]
  14. Kim S.-J., Weon H.-Y., Kim Y.-S., Anandham R., Yoo S.-H., Park I.-C., Kwon S.-W. ( 2010 ). Kaistia terrae sp. nov., isolated from a wetland in Korea. . Int J Syst Evol Microbiol 60, 949952. [View Article] [PubMed]
    [Google Scholar]
  15. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . London:: Wiley;.
    [Google Scholar]
  16. Lee H.-W., Yu H.-S., Liu Q.-M., Jung H.-M., An D.-S., Im W.-T., Jin F.-X., Lee S.-T. ( 2007 ). Kaistia granuli sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. . Int J Syst Evol Microbiol 57, 22802283. [View Article] [PubMed]
    [Google Scholar]
  17. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  18. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [View Article]
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva : a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  20. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  21. Weon H.-Y., Lee C.-M., Hong S.-B., Kim B.-Y., Yoo S.-H., Kwon S.-W., Go S.-J. ( 2008 ). Kaistia soli sp. nov., isolated from a wetland in Korea. . Int J Syst Evol Microbiol 58, 15221524. [View Article] [PubMed]
    [Google Scholar]
  22. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049619-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049619-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error