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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_9

sp. nov., isolated from surface water Free

Abstract

A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria from surface water, was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence similarity comparisons revealed that strain FSt3A was grouped in the genus , most closely related to XDB9 (98.1 %), BAM-582 and DSM 28 (both 98.0 %). The 16S rRNA gene sequence similarity to other species of the genus was <97.5 %. The allocation to the genus was supported by a detailed chemotaxonomic characterization revealing a cell wall containing alanine, glutamic acid, aspartic acid and the diagnostic diamino acid lysine in a molar ratio of 1.6 : 1 : 0.9 : 0.8 (peptidoglycan type A4α), the major menaquinones MK-7 and MK-6, and polar lipids consisting of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unknown phospholipids, one unknown aminophospholipid and one unidentified aminolipid. The major fatty acids were iso- and anteiso-branched fatty acids. DNA–DNA hybridizations with the type strains of the most closely related species, DSM 25242, DSM 23493 and DSM 28, in addition to the results of physiological and biochemical tests, allowed genotypic and phenotypic differentiation of strain FSt3A from these related species. Thus, FSt3A represents a novel species of the genus , for which the name sp. nov. is proposed, with FSt3A ( = CCM 8383 = DSM 25560 = CIP 110362) as the type strain.

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2013-09-01
2025-03-24
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Lysinibacilluscontaminans sp. nov., isolated from surface water
Int J Syst Evol Microbiol 63, 3148 (2013); https://doi.org/10.1099/ijs.0.049593-0
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