1887

Abstract

A Gram-stain-positive, novel actinobacterium, designated strain JL-6, was isolated from the litter of a bamboo () forest in Damyang, Korea. Strain JL-6 had white‐grey, smooth, cylindrical spores that were borne in straight, long spore-chains. The novel strain grew aerobically at 15–28 °C (optimum, 28 °C), pH 4.0–8.0 (optimum, pH 5.5) and with 0–1.5 % (w/v) NaCl. The cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid, alanine and glycine. The predominant menaquinones were MK-9(H) and MK-9(H). Whole-cell hydrolysates mainly contained glucose and ribose. Phosphatidylinositol and phosphatidylcholine were the diagnostic phospholipids. The GC content of the genomic DNA was 72.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JL-6 belonged to the genus with sequence similarities ranging from 97.3 % to 98.3 %. However, DNA–DNA hybridization between JL-6 and the closest related strain, turgidiscabies , ATCC 700248 and other closely related species in the genus showed <50 % relatedness. Based on these observations, strain JL-6 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JL-6 ( = KACC 16470 = NBRC 108882).

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2014-02-01
2020-01-22
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