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Volume 63, Issue Pt_11

sp. nov., isolated from the interfacial sediment of a eutrophic lake Free

Abstract

A novel actinobacterial strain, 3-wff-81, was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain 3-wff-81 belonged to the genus , with PB34 (99.1 % similarity) and PB261 (98.3 % similarity) as closest relatives. The major fatty acids were 16 : 0 iso, 15 : 0 iso, 17 : 1ω8 and 14 : 0 iso. The predominant menaquinones were MK-9(H) and MK-9. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 73.2 mol%. DNA–DNA relatedness values with PB34 and PB261 were 42.8 % and 39.6 %, respectively. Based on the physiological, biochemical and chemotaxonomic data, it is proposed that strain 3-wff-81 represents a novel species named sp. nov. with 3-wff-81 ( = CGMCC 1.12303 = NBRC 109416) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • Chinese National Natural Science Foundation (Award 31271948 and 31000065)
  • Plan for Scientific Innovation Talent of Henan University of Technology (Award 11CXRC14)
  • Doctoral Foundation of Henan University of Technology (Award 2010BS008)
© 2013 IUMS
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2013-11-01
2024-04-24
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Geodermatophilustaihuensis sp. nov., isolated from the interfacial sediment of a eutrophic lake
Int J Syst Evol Microbiol 63, 4108 (2013); https://doi.org/10.1099/ijs.0.049460-0
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