1887

Abstract

A novel actinobacterial strain, 3-wff-81, was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain 3-wff-81 belonged to the genus , with PB34 (99.1 % similarity) and PB261 (98.3 % similarity) as closest relatives. The major fatty acids were 16 : 0 iso, 15 : 0 iso, 17 : 1ω8 and 14 : 0 iso. The predominant menaquinones were MK-9(H) and MK-9. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 73.2 mol%. DNA–DNA relatedness values with PB34 and PB261 were 42.8 % and 39.6 %, respectively. Based on the physiological, biochemical and chemotaxonomic data, it is proposed that strain 3-wff-81 represents a novel species named sp. nov. with 3-wff-81 ( = CGMCC 1.12303 = NBRC 109416) as the type strain.

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2013-11-01
2020-01-29
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