1887

Abstract

Three Gram-staining-negative, rod-shaped, non-spore-forming, non-motile, oxidase-positive, yellow pigmented and aerobic bacterial isolates designated 8_R23573, 9_R23581 and 10_R23577 were isolated from raw chicken at a broiler processing plant in Bloemfontein, South Africa. A polyphasic taxonomic approach was used to determine their exact taxonomic identities. Phylogenetic analysis of the 16S rRNA gene sequences showed that the three strains belonged to the genus , exhibiting the highest similarities to DSM 17126 (98.6–99.2 %) and DSM 18605 (98.3–98.7 %). The most abundant quinone was menaquinone MK-6 and the predominant cellular fatty acids were iso-15 : 0, iso-17 : 1ω9, iso-17 : 0 3-OH and summed feature 3 (iso-16 : 1ω7 and/or iso-15 : 0 2-OH), which supported the affiliation of the strains to the genus . The DNA G+C contents of the strains were 36.9, 36.7 and 36.6 mol% respectively. The DNA–DNA hybridization results gave relatedness values ranging from 78.8 to 87.2 % among the three strains and 23.4 to 56.1 % to the two nearest phylogenetic neighbours DSM 17126 and LMG 23785. On the basis of the data from this polyphasic study, the three strains are concluded to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is 9_R23581 ( = LMG 26732 = DSM 25581).

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2013-09-01
2019-10-14
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