sp. nov., a novel yeast species isolated from black olive brine Free

Abstract

A yeast strain was isolated from olive brines in a fermented black olive and olive oil manufacturing plant in the town of Nyons (France). On the basis of domains 1 and 2 (D1/D2) large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analyses, the strain CLIB 1303 was found to be closely related, but clearly distinct, from the three existing species of the genus : , and . Strain CLIB 1303 exhibited 6 bp, 7 bp and 12 bp divergences in the D1/D2 LSU rRNA gene with and , respectively. ITS region divergence amounted to more than 8 %, 4 % and 4.5 % with and , respectively, in addition to several indels. Like and strains, strain CLIB 1303 was shown to be halotolerant and osmotolerant. Phenotypically, strain CLIB 1303 can be distinguished from other species of the genus by its inability to assimilate trehalose. The strain CLIB 1303 ( = CBS 12700) was assigned to a novel species, sp. nov.

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2013-08-01
2024-03-29
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