1887

Abstract

A novel endophytic actinobacterium, designated strain YIM 68236, was isolated from healthy leaves of . and characterized by using a polyphasic approach. Cells of this strain occurred singly, in pairs or in tetrads. It grew at 10–45 °C, at pH 5.0–8.0 (optimum pH 7.0) and in the presence of 0–3 % (w/v) NaCl. The DNA G+C content was 71.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 68236 belongs to the genus . However, it differed from its closest relatives, DSM 4725, DSM 44509 and DSM 19597 in many phenotypic characteristics. Moreover, the DNA–DNA relatedness values between the novel isolate and the three above-mentioned type strains were 49.0±1.6 %, 46.1±3.2 % and 39.8±1.5 %, respectively. Based on comparative analysis of physiological and chemotaxonomic data, strain YIM 68236 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 68236 ( = CCTCC AA 209045 = DSM 45413 = KCTC 19998).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049239-0
2013-09-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3269.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049239-0&mimeType=html&fmt=ahah

References

  1. Ahrens R., Moll G.. ( 1970;). Ein neues knospendes Bakterium aus der Ostsee. . Arch Mikrobiol 70:, 243–265. (in German). [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  9. Hasegawa T., Takizawa M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  10. He L., Li W., Huang Y., Wang L. M., Liu Z. H., Lanoot B. J., Vancanneyt M., Swings J.. ( 2005;). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. . Int J Syst Evol Microbiol 55:, 1939–1944. [CrossRef][PubMed]
    [Google Scholar]
  11. Jiang Y., Tang S. K., Wiese J., Xu L. H., Imhoff J. F., Jiang C. L.. ( 2007;). Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces. . Int J Syst Evol Microbiol 57:, 2694–2698. [CrossRef][PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  13. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  14. Lee S. D.. ( 2006;). Blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus Blastococcus Ahrens and Moll 1970. . Int J Syst Evol Microbiol 56:, 2391–2396. [CrossRef][PubMed]
    [Google Scholar]
  15. Leifson E.. ( 1960;). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  16. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  17. Li J., Zhao G. Z., Chen H. H., Wang H. B., Qin S., Zhu W. Y., Xu L. H., Jiang C. L., Li W. J.. ( 2008;). Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest. . Lett Appl Microbiol 47:, 574–580. [CrossRef][PubMed]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  21. Skerman V. B. D.. ( 1967;). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  22. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  23. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  25. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J.. ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. . Int J Syst Evol Microbiol 59:, 2025–2032. [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  27. Urzì C., Salamone P., Schumann P., Rohde M., Stackebrandt E.. ( 2004;). Blastococcus saxobsidens sp. nov., and emended descriptions of the genus Blastococcus Ahrens and Moll 1970 and Blastococcus aggregatus Ahrens and Moll 1970. . Int J Syst Evol Microbiol 54:, 253–259. [CrossRef][PubMed]
    [Google Scholar]
  28. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049239-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049239-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error