1887

Abstract

A novel endophytic actinobacterium, designated strain YIM 68236, was isolated from healthy leaves of . and characterized by using a polyphasic approach. Cells of this strain occurred singly, in pairs or in tetrads. It grew at 10–45 °C, at pH 5.0–8.0 (optimum pH 7.0) and in the presence of 0–3 % (w/v) NaCl. The DNA G+C content was 71.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 68236 belongs to the genus . However, it differed from its closest relatives, DSM 4725, DSM 44509 and DSM 19597 in many phenotypic characteristics. Moreover, the DNA–DNA relatedness values between the novel isolate and the three above-mentioned type strains were 49.0±1.6 %, 46.1±3.2 % and 39.8±1.5 %, respectively. Based on comparative analysis of physiological and chemotaxonomic data, strain YIM 68236 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 68236 ( = CCTCC AA 209045 = DSM 45413 = KCTC 19998).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award U0932601)
  • Basic Research Foundation of Yunnan University (Award 2010ZD001)
  • Chinese Academy of Sciences
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2013-09-01
2021-07-28
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