A Gram-staining-positive actinobacterial strain, Chem10T, was isolated from soil around Inche-Broun hypersaline wetland in the north of Iran. Strain Chem10T was strictly aerobic, and catalase- and oxidase-positive. The isolate grew with 0–3 % NaCl, at 20–40 °C and at pH 6.0–8.0. The optimum temperature and pH for growth were 30 °C and pH 7.0, respectively. The cell wall of strain Chem10T contained meso-diaminopimelic acid as diamino acid and galactose, ribose and arabinose as whole-cell sugars. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain Chem10T synthesized cellular fatty acids of the straight-chain saturated and mono-unsaturated, and iso- and anteiso-branched types C14 : 0, C16 : 0, iso-C16 : 1, anteiso-C17 : 0, iso-C16 : 0, iso-C14 : 0 and iso-C15 : 0, and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Chem10T belonged to the family Pseudonocardiaceae and showed the closest phylogenetic similarity to Alloactinosynnema album KCTC 19294T (98.3 %) and Actinokineospora cibodasensis DSM 45658T (97.9 %). DNA–DNA relatedness values between the novel strain and strains Alloactinosynnema album KCTC 19294T and Actinokineospora cibodasensis DSM 45658T were only 52 % and 23 %, respectively. On the basis of phylogenetic analysis, phenotypic characteristics and DNA–DNA hybridization data, a novel species of the genus Alloactinosynnema is proposed, Alloactinosynnema iranicum sp. nov. The type strain is Chem10T ( = IBRC-M 10403T = CECT 8209T). In addition, an emended description of the genus Alloactinosynnema is proposed.
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Funding
This study was supported by the:
Iranian Biological Resource Centre (IBRC)
(Award MI-1391-15)
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