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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_4

sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Free

Abstract

A Gram-staining-positive actinobacterial strain, Chem10, was isolated from soil around Inche-Broun hypersaline wetland in the north of Iran. Strain Chem10 was strictly aerobic, and catalase- and oxidase-positive. The isolate grew with 0–3 % NaCl, at 20–40 °C and at pH 6.0–8.0. The optimum temperature and pH for growth were 30 °C and pH 7.0, respectively. The cell wall of strain Chem10 contained -diaminopimelic acid as diamino acid and galactose, ribose and arabinose as whole-cell sugars. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain Chem10 synthesized cellular fatty acids of the straight-chain saturated and mono-unsaturated, and iso- and anteiso-branched types C, C, iso-C, anteiso-C, iso-C, iso-C and iso-C, and the major respiratory quinone was MK-9(H). The G+C content of the genomic DNA was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Chem10 belonged to the family and showed the closest phylogenetic similarity to KCTC 19294 (98.3 %) and DSM 45658 (97.9 %). DNA–DNA relatedness values between the novel strain and strains KCTC 19294 and DSM 45658 were only 52 % and 23 %, respectively. On the basis of phylogenetic analysis, phenotypic characteristics and DNA–DNA hybridization data, a novel species of the genus is proposed, sp. nov. The type strain is Chem10 ( = IBRC-M 10403 = CECT 8209). In addition, an emended description of the genus is proposed.

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Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1391-15)
© 2014 IUMS
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2014-04-01
2025-01-25
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Alloactinosynnema iranicum sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Alloactinosynnema
Int J Syst Evol Microbiol 64, 1173 (2014); https://doi.org/10.1099/ijs.0.049189-0
/content/journal/ijsem/10.1099/ijs.0.049189-0
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