1887

Abstract

A Gram-staining-positive actinobacterial strain, Chem10, was isolated from soil around Inche-Broun hypersaline wetland in the north of Iran. Strain Chem10 was strictly aerobic, and catalase- and oxidase-positive. The isolate grew with 0–3 % NaCl, at 20–40 °C and at pH 6.0–8.0. The optimum temperature and pH for growth were 30 °C and pH 7.0, respectively. The cell wall of strain Chem10 contained -diaminopimelic acid as diamino acid and galactose, ribose and arabinose as whole-cell sugars. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain Chem10 synthesized cellular fatty acids of the straight-chain saturated and mono-unsaturated, and iso- and anteiso-branched types C, C, iso-C, anteiso-C, iso-C, iso-C and iso-C, and the major respiratory quinone was MK-9(H). The G+C content of the genomic DNA was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Chem10 belonged to the family and showed the closest phylogenetic similarity to KCTC 19294 (98.3 %) and DSM 45658 (97.9 %). DNA–DNA relatedness values between the novel strain and strains KCTC 19294 and DSM 45658 were only 52 % and 23 %, respectively. On the basis of phylogenetic analysis, phenotypic characteristics and DNA–DNA hybridization data, a novel species of the genus is proposed, sp. nov. The type strain is Chem10 ( = IBRC-M 10403 = CECT 8209). In addition, an emended description of the genus is proposed.

Funding
This study was supported by the:
  • , Iranian Biological Resource Centre (IBRC) , (Award MI-1391-15)
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2014-04-01
2020-07-04
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