1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_11

sp. nov., isolated from the larval gut of (Diptera: Stratiomyidae), and emended description of the genus Free

Abstract

A novel Gram-stain-negative, facultatively anaerobic, non-motile and short rod-shaped bacterium, strain KBL009, was isolated from the larval gut of . Strain KBL009 grew optimally at 37 °C, at pH 6.0 and with 1–2 % (w/v) NaCl. The 16S rRNA gene sequence of strain KBL009 showed 97.6 % similarity to that of CCUG 53761A indicating its classification with the genus . The major fatty acids were cyclo-C, C and summed feature 2 (comprising C 3-OH/iso-C). The respiratory quinones were ubiquinone-8 (Q-8), predominating, and a minor amount of Q-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unknown aminolipid and five unknown polar lipids. The polyamine pattern contained predominantly putrescine and relatively high amounts of spermidine. The betaproteobacterial-specific 2-hydroxyputrescine could only be detected in trace amounts. The G+C content of genomic DNA was 56.1 mol%. Results from DNA–DNA hybridization with KCTC 23583 unambiguously demonstrated that strain KBL009 represents a novel species in the genus . Based on phenotypic, genotypic and phylogenetic characterization, the novel species sp. nov. is proposed. The type strain is KBL009 ( = KACC 16840 = JCM 18423). An emended description of the genus is also provided.

  • Published Online:
Funding
This study was supported by the:
  • Rural Development Administration in Korea (Award PJ006893)
  • National
  • Research Foundation of Korea (NRF) (Award 2010-0010779)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049098-0
2013-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4224.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049098-0&mimeType=html&fmt=ahah

References

  1. Brauman A., Doré J., Eggleton P., Bignell D., Breznak J. A., Kane M. D. ( 2001 ). Molecular phylogenetic profiling of prokaryotic communities in guts of termites with different feeding habits. . FEMS Microbiol Ecol 35, 27–36. [View Article] [PubMed]
    [Google Scholar]
  2. Breznak J. A. ( 1982 ). Intestinal microbiota of termites and other xylophagous insects. . Annu Rev Microbiol 36, 323–343. [View Article] [PubMed]
    [Google Scholar]
  3. Busse J., Auling G. ( 1988 ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11, 1–8. [View Article]
    [Google Scholar]
  4. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. ( 2008 ). Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75, 523–530. [View Article] [PubMed]
    [Google Scholar]
  5. Collins M. D., Jones D. ( 1981a ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316–354.[PubMed]
    [Google Scholar]
  6. Collins M. D., Jones D. ( 1981b ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51, 129–134. [View Article] [PubMed]
    [Google Scholar]
  7. Diener S., Zurbrügg C., Tockner K. ( 2009 ). Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates. . Waste Manag Res 27, 603–610. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  9. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 95–98.
     [Google Scholar]
  10. Hamana K., Takeuchi M. ( 1998 ). Polyamine profiles as chemotaxonomic markers within alpha, beta, gamma, delta, and epsilon subclasses of class Proteobacteria: distribution of 2-hydroxyputrescine and homospermidine. . Microbiol Cult Collect 14, 1–14.
     [Google Scholar]
  11. Hamana K., Saito T., Okada M. ( 2000 ). Polyamine profiles within the beta subclass of the class Proteobacteria: distribution of 2-hydroxyputrescine. . Microbiol Cult Collect 16, 63–69.
     [Google Scholar]
  12. Hamana K., Sato W., Gouma K., Yu J., Ino Y., Umemura Y., Mochizuki C., Takatsuka X., Kigure Y. & other authors ( 2006 ). Cellular polyamine catalogues of the five classes of the phylum Proteobacteria: distributions of homospermidine within the class Alphaproteobacteria, hydroxyputrescine within the class Betaproteobacteria, norspermidine within the class Gammaproteobacteria, and spermidine within the classes Deltaproteobacteria and Epsilonproteobacteria . . Ann Gunma Health Sci 27, 1–16.
     [Google Scholar]
  13. Hongoh Y., Ohkuma M., Kudo T. ( 2003 ). Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae). . FEMS Microbiol Ecol 44, 231–242. [View Article] [PubMed]
    [Google Scholar]
  14. Jeon H., Park S., Choi J., Jeong G., Lee S.-B., Choi Y., Lee S.-J. ( 2011 ). The intestinal bacterial community in the food waste-reducing larvae of Hermetia illucens . . Curr Microbiol 62, 1390–1399. [View Article] [PubMed]
    [Google Scholar]
  15. Kamekura M. ( 1993 ). Lipids of extreme halophiles. . In The Biology of Halophilic Bacteria, pp. 135–161. Edited by Vreeland R. H., Hochstein L. I. . Boca Raton:: CRC Press;.
     [Google Scholar]
  16. Kämpfer P., Falsen E., Langer S., Lodders N., Busse H.-J. ( 2010 ). Paenalcaligenes hominis gen. nov., sp. nov., a new member of the family Alcaligenaceae . . Int J Syst Evol Microbiol 60, 1537–1542. [View Article] [PubMed]
    [Google Scholar]
  17. Kim J. G., Choi Y. C., Choi J. Y., Kim W. T., Jeong G. S., Park K. H., Hwang S. J. ( 2008 ). Ecology of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae) in Korea. . Korean J Appl Entomol 47, 337–343. (Korean). [View Article]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [CrossRef]
    [Google Scholar]
  19. Kim J. Y., Lee J., Shin N.-R., Yun J.-H., Whon T. W., Kim M.-S., Jung M.-J., Roh S. W., Hyun D.-W., Bae J.-W. ( 2013 ). Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus . . Int J Syst Evol Microbiol 63, 1766–1770. [View Article] [PubMed]
    [Google Scholar]
  20. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 1–32. [View Article]
    [Google Scholar]
  21. Kudo T. ( 2009 ). Termite-microbe symbiotic system and its efficient degradation of lignocellulose. . Biosci Biotechnol Biochem 73, 2561–2567. [View Article] [PubMed]
    [Google Scholar]
  22. Loy A., Schulz C., Lücker S., Schöpfer-Wendels A., Stoecker K., Baranyi C., Lehner A., Wagner M. ( 2005 ). 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales”. . Appl Environ Microbiol 71, 1373–1386. [View Article] [PubMed]
    [Google Scholar]
  23. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297–306. [View Article] [PubMed]
    [Google Scholar]
  24. MIDI ( 1999 ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  25. Ohkuma M. ( 2008 ). Symbioses of flagellates and prokaryotes in the gut of lower termites. . Trends Microbiol 16, 345–352. [View Article] [PubMed]
    [Google Scholar]
  26. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. ( 1992 ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79, 59–65.[PubMed] [CrossRef]
    [Google Scholar]
  27. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  28. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  29. Schmitt-Wagner D., Friedrich M. W., Wagner B., Brune A. ( 2003 ). Phylogenetic diversity, abundance, and axial distribution of bacteria in the intestinal tract of two soil-feeding termites (Cubitermes spp.). . Appl Environ Microbiol 69, 6007–6017. [View Article] [PubMed]
    [Google Scholar]
  30. Stolz A., Bürger S., Kuhm A., Kämpfer P., Busse H.-J. ( 2005 ). Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates. . Int J Syst Evol Microbiol 55, 1077–1081. [View Article] [PubMed]
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 4673–4680. [View Article] [PubMed]
    [Google Scholar]
  33. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  34. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575–580.[PubMed]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  36. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697–703.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049098-0
Loading
Paenalcaligenes hermetiae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae), and emended description of the genus Paenalcaligenes
Int J Syst Evol Microbiol 63, 4224 (2013); https://doi.org/10.1099/ijs.0.049098-0
/content/journal/ijsem/10.1099/ijs.0.049098-0
/content/journal/ijsem/10.1099/ijs.0.049098-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error