1887

Abstract

A novel Gram-stain-negative, facultatively anaerobic, non-motile and short rod-shaped bacterium, strain KBL009, was isolated from the larval gut of . Strain KBL009 grew optimally at 37 °C, at pH 6.0 and with 1–2 % (w/v) NaCl. The 16S rRNA gene sequence of strain KBL009 showed 97.6 % similarity to that of CCUG 53761A indicating its classification with the genus . The major fatty acids were cyclo-C, C and summed feature 2 (comprising C 3-OH/iso-C). The respiratory quinones were ubiquinone-8 (Q-8), predominating, and a minor amount of Q-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unknown aminolipid and five unknown polar lipids. The polyamine pattern contained predominantly putrescine and relatively high amounts of spermidine. The betaproteobacterial-specific 2-hydroxyputrescine could only be detected in trace amounts. The G+C content of genomic DNA was 56.1 mol%. Results from DNA–DNA hybridization with KCTC 23583 unambiguously demonstrated that strain KBL009 represents a novel species in the genus . Based on phenotypic, genotypic and phylogenetic characterization, the novel species sp. nov. is proposed. The type strain is KBL009 ( = KACC 16840 = JCM 18423). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , Rural Development Administration in Korea , (Award PJ006893)
  • , National
  • , Research Foundation of Korea (NRF) , (Award 2010-0010779)
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2013-11-01
2020-12-03
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