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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_8

sp. nov., a phototrophic bacterium isolated from rhizosphere soil of paddy Free

Abstract

A reddish-brown bacterium, designated strain JA318, was purified from a photoheterotrophic enrichment culture obtained from the rhizosphere soil of paddy. Cells of strain JA318 are spiral shaped, Gram-stain-negative and motile by means of amphitrichous flagella. Strain JA318 has no NaCl requirement for growth but can tolerate up to 1.5 % (w/v) NaCl. Internal photosynthetic membranes are present as lamellar stacks. Photoorganoheterotrophy is the only growth mode observed. Strain JA318 contains bacteriochlorophyll , lycopene and rhodopin as major carotenoids. Thiamine, niacin and -aminobenzoic acid (PABA) are required as growth factors. Major fatty acids are Cω7 and C. Ubiquinone-8 and rhodoquinone-8 are the observed quinones. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid are the major polar lipids in strain JA318. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain JA318 clustered with species of the genus which belongs to the class . The highest sequence similarity of strain JA318 was found with JA143 (99.9 %). The DNA–DNA reassociation values of strain JA318 with JA143 and DSM 122 were 52±2 % and 45±1 %, respectively. The genomic DNA G+C content of strain JA318 was 60.2 mol%. Based on the morphological, physiological, chemotaxonomical and molecular evidence, strain JA318 is significantly different from the type strains of species of the genus , of the family , and it is proposed that the strain be classified as a representative of a novel species for which the name sp. nov. is proposed. The type strain is JA318 ( = KCTC 5960 = NBRC 107573).

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Funding
This study was supported by the:
  • Council of Scientific and Industrial Research CSIR
© 2013 IUMS
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2013-08-01
2025-01-18
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Rhodospirillumoryzae sp. nov., a phototrophic bacterium isolated from rhizosphere soil of paddy
Int J Syst Evol Microbiol 63, 3050 (2013); https://doi.org/10.1099/ijs.0.049023-0
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