1887

Abstract

A novel strain, designated W-15, was isolated from the gut of a long-horned beetle, , collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder . Strain W-15 was most closely related to RI148-Li105 (97.9 % similarity). Strain W-15 was Gram-stain-positive, rod- and coccus-shaped and non-motile. Growth was observed at 15–37 °C, at pH 4.5–8.5 and in the presence of 0–5.0 % NaCl. The cell-wall peptidoglycan of the strain was A4α (-Lys–-Ser–-Asp). The major menaquinone present in this strain was MK-8 (H) and the major cellular fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown lipid, an unknown phospholipid and an unknown phosphoglycolipid. The G+C content of genomic DNA of the strain was 73.8 mol%. On the basis of evidence from our polyphasic taxonomic study, strain W-15 is classified as representing a novel species in the suborder , for which the name sp. nov. is proposed. The type strain of this species is strain W-15 ( = KCTC 19882 = JCM 18090).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.048991-0
2014-04-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1401.html?itemId=/content/journal/ijsem/10.1099/ijs.0.048991-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Hamada M., Otoguro M., Yamamura H., Tamura T., Suzuki K., Hayakawa M.. ( 2010;). Luteimicrobium subarcticum gen. nov., sp. nov., a new member of the suborder Micrococcineae. . Int J Syst Evol Microbiol 60:, 796–800. [CrossRef][PubMed]
    [Google Scholar]
  5. Hamada M., Yamamura H., Komukai C., Tamura T., Suzuki K., Hayakawa M.. ( 2012;). Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. . J Antibiot (Tokyo) 65:, 427–431. [CrossRef][PubMed]
    [Google Scholar]
  6. Kim H., Park D. S., Oh H. W., Lee K. H., Chung D. H., Park H. Y., Park H. M., Bae K. S.. ( 2012;). Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov.. Int J Syst Evol Microbiol 62:, 2363–2370. [CrossRef][PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  8. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  9. Sambrook J., Fritsch E. F., Maniatis T.. ( 1989;). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  10. Sasser M.. ( 2001;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  11. Schumann P.. ( 2011;). Peptidoglycan structure. . Methods Microbiol 38:, 101–129. [CrossRef]
    [Google Scholar]
  12. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  13. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.048991-0
Loading
/content/journal/ijsem/10.1099/ijs.0.048991-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error