1887

Abstract

A novel bacterial strain, YC6899, was isolated from the root of growing on a tidal flat of Namhae Island, Korea. Cells were Gram-reaction-negative, rod-shaped, non-motile, slightly halophilic and heterotrophic. Strain YC6899 grew optimally at a salinity of 2–4 %, at 25–37 °C and at pH 6.5–8.0. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that strain YC6899 was closely related to YIM D10 (96.1 % similarity) and RS.Sph.026 (95.9 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown aminolipids, an unknown aminophospholipid, an unknown aminoglycolipid, unknown glycolipids and unknown lipids. The major fatty acids of strain YC6899 were C cyclo ω8 and Cω7. The G+C content of the genomic DNA was 61.3 mol%. Strain YC6899 contained ubiquinone-10 (Q-10) as the major respiratory quinone system. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YC6899 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is YC6899 ( = KACC 14912 = NBRC 107845).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.048959-0
2013-10-01
2020-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/10/3868.html?itemId=/content/journal/ijsem/10.1099/ijs.0.048959-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 1993;). Handbook of Microbiological Media. Edited by Parks L. C... Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Bibi F., Yasir M., Song G. C., Lee S. Y., Chung Y. R.. ( 2012;). Diversity and characterization of endophytic bacteria associated with tidal flat plants and their antagonistic effects on oomycetous plant pathogens. . Plant Pathol J 28:, 20–31. [CrossRef]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N.. ( 2002;). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  5. Chung B. S., Aslam Z., Kim S. W., Kim G. G., Kang H. S., Ahn J. W., Chung Y. R.. ( 2008;). A bacterial endophyte, Pseudomonas brassicacearum YC5480 isolated from the root of Artemisia sp. producing antifungal and phytotoxic compounds. . Plant Pathol J 24:, 461–468. [CrossRef]
    [Google Scholar]
  6. Dittmer J. C., Lester R. L.. ( 1964;). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res 5:, 126–127.[PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M.. ( 1972;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. M., Wood W. A., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  11. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequencealignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  12. Hendricks C. W., Doyle J. D., Hugley B.. ( 1995;). A new solid medium for enumerating cellulose-utilizing bacteria in soil. . Appl Environ Microbiol 61:, 2016–2019. [CrossRef][PubMed]
    [Google Scholar]
  13. Jacin H., Mishkin A. R.. ( 1965;). Separation of carbohydrates on borate impregnated silica gel G plates. . J Chromatogr A 18:, 170–173. [CrossRef][PubMed]
    [Google Scholar]
  14. Kim H., Choo Y. J., Cho J. C.. ( 2007;). Litoricolaceae fam. nov., to include Litoricola lipolytica gen. nov., sp. nov., a marine bacterium belonging to the order Oceanospirillales. . Int J Syst Evol Microbiol 57:, 1793–1798. [CrossRef][PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K. H., Yoon S. H., Kim M. C., Na H. S., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  17. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... New York:: Wiley;.
    [Google Scholar]
  18. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  21. Ross H. N. M., Grant W. D., Harris J. E.. ( 1985;). Lipids in archaebacterial taxonomy. . In Chemical Methods in Bacterial Systematics, pp. 289–300. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Shieh W. Y., Chen Y. W., Chaw S. M., Chiu H. H.. ( 2003;). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. . Int J Syst Evol Microbiol 53:, 479–484. [CrossRef][PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Ten L. N., Im W. T., Kim M. K., Kang M. S., Lee S. T.. ( 2004;). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. . J Microbiol Methods 56:, 375–382. [CrossRef][PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  28. Wang Y. X., Liu J. H., Chen Y. G., Zhang X. X., Wang Z. G., Chen Y., Tian S. P., Hu B., Cui X. L.. ( 2010;). Amorphus orientalis sp. nov., an exopolysaccharide-producing bacterium isolated from salt mine sediment. . Int J Syst Evol Microbiol 60:, 1750–1754. [CrossRef][PubMed]
    [Google Scholar]
  29. Zeevi Ben Yosef D., Ben-Dov E., Kushmaro A.. ( 2008;). Amorphus coralli gen. nov., sp. nov., a marine bacterium isolated from coral mucus, belonging to the order Rhizobiales. . Int J Syst Evol Microbiol 58:, 2704–2709. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.048959-0
Loading
/content/journal/ijsem/10.1099/ijs.0.048959-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error