RT Journal Article SR Electronic(1) A1 Yang, Guiqin A1 Zhou, Xuemei A1 Zhou, Shungui A1 Yang, Dehui A1 Wang, Yueqiang A1 Wang, DingmeiYR 2013 T1 Bacillusthermotolerans sp. nov., a thermophilic bacterium capable of reducing humus JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_10 SP 3672 OP 3678 DO https://doi.org/10.1099/ijs.0.048942-0 PB Microbiology Society, SN 1466-5034, AB A novel thermotolerant bacterium, designated SgZ-8T, was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalase-positive. The isolate was able to grow at 20–65 °C (optimum 50 °C) and pH 6.0–9.0 (optimum 6.5–7.0), and tolerate up to 9.0 % NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO3 - as electron acceptors. Phylogenetic analysis based on the16S rRNA and gyrB genes grouped strain SgZ-8T into the genus Bacillus , with the highest similarity to Bacillus badius JCM 12228T (96.2 % for 16S rRNA gene sequence and 83.5 % for gyrB gene sequence) among all recognized species in the genus Bacillus . The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C16 : 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8T ( = CCTCC AB 2012108T = KACC 16706T) was designated the type strain of a novel species of the genus Bacillus , for which the name Bacillus thermotolerans sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.048942-0