1887

Abstract

A novel thermotolerant bacterium, designated SgZ-8, was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalase-positive. The isolate was able to grow at 20–65 °C (optimum 50 °C) and pH 6.0–9.0 (optimum 6.5–7.0), and tolerate up to 9.0 % NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO as electron acceptors. Phylogenetic analysis based on the16S rRNA and B genes grouped strain SgZ-8 into the genus , with the highest similarity to JCM 12228 (96.2 % for 16S rRNA gene sequence and 83.5 % for B gene sequence) among all recognized species in the genus . The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8 ( = CCTCC AB 2012108 = KACC 16706) was designated the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31070460, 21177030 and 41171205)
  • Strategic Cooperation Research of Chinese Academy of Sciences and Guangdong Province (Award 2011A090200038 and 2011B090300076)
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2013-10-01
2021-10-16
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