1887

Abstract

A Gram-staining-negative, non-spore-forming endophytic bacterium, designated strain YC6887, was isolated from a root sample of a halophyte, , collected from a tidal flat area of Namhae Island, located at the southern end of Korea. Strain YC6887 was found to exhibit inhibitory activity against oomycete plant pathogens. The cells were non-motile and aerobic rods. The strain was able to grow at 4–40 °C (optimum 28–30 °C) and at pH 5.0–9.0 (optimum pH 7.0–8.5). Strain YC6887 was able to grow at NaCl concentrations of 0–9 % (w/v) with optimum growth at 4–5 % (w/v) NaCl, but NaCl is not essential for growth. Comparison of 16S rRNA gene sequences showed that the strain was a member of the genus , a member of order , exhibiting highest similarity with (98.6 %). The DNA–DNA relatedness between strain YC6887 and MACL11 was 19.8±6.8. Chemotaxonomically, strain YC6887 contained C cyclo ω8 (28.0 %) and Cω7 (17.9 %) as predominant fatty acids, confirming the affiliation of strain YC6887 with the genus . The major respiratory quinone was Q-10 and the DNA G+C content was 62.1 mol%. On the basis of phylogenetic analysis, physiological and biochemical characterization and DNA–DNA hybridization data, strain YC6887 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC6887 ( = KCCM 43011 = NBRC 109149).

Funding
This study was supported by the:
  • , Brain Korea (BK) 21 project
  • , Ministry of Education, Science and Technology, Korea
  • , Ministry of Food, Agriculture, Forestry and Fisheries , (Award 808015-3)
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2013-08-01
2020-06-01
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