1887

Abstract

A Gram-staining-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, HD-3, was isolated from a tidal flat sediment of Hwangdo in the Yellow Sea, South Korea. Strain HD-3 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HD-3 joined the cluster comprising the type strains of and . Strain HD-3 exhibited 16S rRNA gene sequence similarity values of 93.8 % to each of the type strains of and . Strain HD-3 contained MK-6 as the only menaquinone and iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH as the major fatty acids. The polar lipid profile of strain HD-3, which contained phosphatidylethanolamine and one unidentified lipid as major components and one unidentified phospholipid as a significant component, differed slightly from those of the type strains of and . The DNA G+C content of strain HD-3 was 42.3 mol%. Differential phenotypic and chemotaxonomic properties and phylogenetic data of strain HD-3 demonstrated that this strain is distinguishable from and . On the basis of the data presented, strain HD-3 is considered to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is HD-3 ( = KCTC 32177 = CCUG 63246).

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2013-09-01
2019-10-20
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References

  1. Bae S. S. , Kwon K. K. , Yang S. H. , Lee H. S. , Kim S. J. , Lee J. H. . ( 2007; ). Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome. . Int J Syst Evol Microbiol 57:, 1050–1054. [CrossRef] [PubMed]
    [Google Scholar]
  2. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  3. Baumann P. , Baumann L. . ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, vol. 1, pp. 1302–1331. Edited by Starr M. P. , Stolp H. , Trüper H. G. , Balows A. , Schlegel H. G. . . Berlin:: Springer;.
    [Google Scholar]
  4. Bernardet J.-F. . ( 2011; ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R. , Ludwig W. , Whitman W. B. , Hedlund B. P. , Paster B. J. , Staley J. T. , Ward N. , Brown D. , Parte A. . . New York:: Springer;.
    [Google Scholar]
  5. Bernardet J.-F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl & Tait 1978). . Int J Syst Bacteriol 46:, 128–148. [CrossRef]
    [Google Scholar]
  6. Bernardet J.-F. , Nakagawa Y. , Holmes B. . ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  7. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  8. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef] [PubMed]
    [Google Scholar]
  9. Cohen-Bazire G. , Sistrom W. R. , Stanier R. Y. . ( 1957; ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef]
    [Google Scholar]
  10. Khan S. T. , Nakagawa Y. , Harayama S. . ( 2008; ). Fulvibacter tottoriensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment. . Int J Syst Evol Microbiol 58:, 1670–1674. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Komagata K. , Suzuki K. . ( 1988; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  13. Lányí B. . ( 1988; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  14. Lee D. H. , Kahng H.-Y. , Lee Y. S. , Jung J. S. , Kim J. M. , Chung B. S. , Park S. K. , Jeon C. O. . ( 2009; ). Jejuia pallidilutea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater. . Int J Syst Evol Microbiol 59:, 2148–2152. [CrossRef] [PubMed]
    [Google Scholar]
  15. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  16. Macián M. C. , Pujalte M. J. , Márquez M. C. , Ludwig W. , Ventosa A. , Garay E. , Schleifer K. H. . ( 2002; ). Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae. . Int J Syst Evol Microbiol 52:, 1325–1329. [CrossRef] [PubMed]
    [Google Scholar]
  17. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  18. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  19. Nedashkovskaya O. I. , Kim S. B. , Lysenko A. M. , Frolova G. M. , Mikhailov V. V. , Bae K. S. . ( 2005; ). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. . Int J Syst Evol Microbiol 55:, 375–378. [CrossRef] [PubMed]
    [Google Scholar]
  20. Reichenbach H. . ( 1992; ). The order Cytophagales . . In The Prokaryotes , , 2nd edn., vol. 4, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  21. Romanenko L. A. , Uchino M. , Frolova G. M. , Mikhailov V. V. . ( 2007; ). Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. . Int J Syst Evol Microbiol 57:, 457–462. [CrossRef] [PubMed]
    [Google Scholar]
  22. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  23. Staley J. T. . ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  24. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  25. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  26. Yoon J.-H. , Lee S. T. , Park Y.-H. . ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  27. Yoon J.-H. , Kang K. H. , Park Y.-H. . ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon B.-J. , Lee D.-H. , Kang B.-J. , Kahng H.-Y. , Oh Y.-S. , Sohn J.-H. , Choi E.-S. , Oh D.-C. . ( 2010; ). Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. . Int J Syst Evol Microbiol 60:, 382–386. [CrossRef] [PubMed]
    [Google Scholar]
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