1887

Abstract

Two stalked, aerobic, catalase- and oxidase-positive rod-shaped isolates, VKM B-1508 ( = CB 258) and FWC47, were analysed using a polyphasic approach. While the morphology and the 16S rRNA gene sequence of strain VKM B-1508 were 100 % identical to the ones of DSM 4733, the morphology of FWC47 was different, and the closest recognized species were S213 ( = DSM 17107) and DSM 4733 with 97.2 % and 97.0 % 16S rRNA gene sequence similarity, respectively. DNA–DNA hybridization studies supported the differentiation of strain FWC47 from and . Strain FWC47 grew optimally at 28–30 °C, and pH 6.0–8.0. The major respiratory quinone was Q10 and the major polyamine was -homospermidine. The major fatty acids were Cω6 and Cω7 and C 2-OH was the major 2-hydroxy fatty acid. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethylamine and unidentified sphingoglycolipids. The G+C content of the genomic DNA of strain FWC47 was 67.1 mol%. Strain FWC47 differed from by its ability to assimilate -alanine, maltose and sucrose, by the presence of β-galactosidase and α-chymotrypsin, and the lack of valine arylamidase and β-glucosidase activities. Contrary to , FWC47 did not reduce nitrate and could not use fructose, acetate and -acetyl-glusosamine. In the genus , the dimorphic life cycle involving a prosthecate sessile and a flagellated swarmer cell was hitherto only known from . Therefore, strain FWC47 represents an additional distinct prosthecate species in this genus for which the name is proposed. The type strain is FWC47 ( = LMG 27141 = CCUG 62982).

Funding
This study was supported by the:
  • German Federal Ministry for Science, Education and Research (Award 0319433C)
  • European Union
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
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2013-09-01
2024-03-29
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