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Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated WT-RY4, was isolated from wood falls in the South Sea, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain WT-RY4 grew optimally at 25 °C, at pH 7.0–7.5 and in the absence of NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WT-RY4 clustered with the type strain of with a bootstrap resampling value of 100 %. Strain WT-RY4 exhibited 16S rRNA gene sequence similarity values of 98.8 % and 96.3 % to RL-2 and IMCC 1704, respectively and less than 91.5 % to the type strains of other species used in the phylogenetic analysis. The DNA G+C content of strain WT-RY4 was 52.4 mol% and the mean DNA–DNA relatedness value with RL-2 was 25 %. Strain WT-RY4 contained Q-11 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), C and C 3-OH as the major fatty acids. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain WT-RY4 was distinguishable from RL-2. On the basis of the data presented, strain WT-RY4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WT-RY4 ( = KCTC 32216 = CCUG 63419). An emended description of the genus is also provided.

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2013-11-01
2020-01-17
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