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Abstract

A bacterial strain designated Npb-07 was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomic approach. Strain Npb-07 was Gram-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 10–37 °C (optimum, 20–30 °C), at pH 6.0–9.0 (optimum, pH 6.0–7.0) and with 0–1 % NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-07 belonged to the genus and its most closely related neighbour was ATCC 19706 with sequence similarity of 98.4 %. The major fatty acids were summed feature 3 (comprising Cω7 and/or Cω6 44.4 %) and C (31.9 %). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 65.3 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two uncharacterized aminophospholipids and an uncharacterized phospholipid. The DNA–DNA relatedness of strain Npb-07 with respect to ATCC 19706 was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Npb-07 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Npb-07 ( = LMG 26669 = BCRC 80377 = KCTC 23713).

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2013-08-01
2020-01-20
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