1887

Abstract

The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, LMG 27206, LMG 27207 and MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these isolates belong to the genus , specifically the group, with and as the nearest neighbours (99.45 and 98.56 % 16S rRNA gene sequence similarity to the respective type strains). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism (FAFLP) and pulsed-field gel electrophoresis (PFGE), DNA–DNA hybridizations, comparative sequence analysis of , and and physiological and biochemical tests revealed that these bacteria formed a taxon well separated from its nearest neighbours and other species of the genus with validly published names and, therefore, represent a novel species, for which the name sp. nov. is proposed, with LMG 27207 ( = DSM 26920) as the type strain.

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2013-11-01
2019-09-22
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References

  1. Arbique J. C. , Poyart C. , Trieu-Cuot P. , Quesne G. , Carvalho M. G. , Steigerwalt A. G. , Morey R. E. , Jackson D. , Davidson R. J. , Facklam R. R. . ( 2004; ). Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov.. J Clin Microbiol 42:, 4686–4696. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bentley R. W. , Leigh J. A. , Collins M. D. . ( 1991; ). Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. . Int J Syst Bacteriol 41:, 487–494. [CrossRef] [PubMed]
    [Google Scholar]
  3. Björkroth J. , Korkeala H. . ( 1996; ). Evaluation of Lactobacillus sake contamination in vacuum-packaged sliced cooked meat products by ribotyping. . J Food Prot 59:, 398–401.
    [Google Scholar]
  4. Bridge P. D. , Sneath P. H. A. . ( 1982; ). Streptococcus gallinarum sp. nov. and Streptococcus oralis sp. nov.. Int J Syst Bacteriol 32:, 410–415. [CrossRef]
    [Google Scholar]
  5. Cleenwerck I. , Vandemeulebroecke K. , Janssens D. , Swings J. . ( 2002; ). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52:, 1551–1558. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridisation in microdilution wells as an alternative to membrane filter hybridisation in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Facklam R. R. . ( 2002; ). What happened to the streptococci: overview of taxonomic and nomenclature changes. . Clin Microbiol Rev 15:, 613–630. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gevers D. , Huys G. , Swings J. . ( 2001; ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. . FEMS Microbiol Lett 205:, 31–36. [CrossRef] [PubMed]
    [Google Scholar]
  9. Glazunova O. O. , Raoult D. , Roux V. . ( 2006; ). Streptococcus massiliensis sp. nov., isolated from a patient blood culture. . Int J Syst Evol Microbiol 56:, 1127–1131. [CrossRef] [PubMed]
    [Google Scholar]
  10. Glazunova O. O. , Raoult D. , Roux V. . ( 2010; ). Partial recN gene sequencing: a new tool for identification and phylogeny within the genus Streptococcus . . Int J Syst Evol Microbiol 60:, 2140–2148. [CrossRef] [PubMed]
    [Google Scholar]
  11. Goris J. , Suzuki K. , De Vos P. , Nakase T. , Kersters K. . ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  12. Gouy M. , Guindon S. P. , Gascuel O. . ( 2010; ). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. . Mol Biol Evol 27:, 221–224. [CrossRef] [PubMed]
    [Google Scholar]
  13. Handley P. , Coykendall A. , Beighton D. , Hardie J. M. , Whiley R. A. . ( 1991; ). Streptococcus crista sp. nov., a viridans streptococcus with tufted fibrils, isolated from the human oral cavity and throat. . Int J Syst Bacteriol 41:, 543–547. [CrossRef] [PubMed]
    [Google Scholar]
  14. Hoshino T. , Fujiwara T. , Kilian M. . ( 2005; ). Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients. . J Clin Microbiol 43:, 6073–6085. [CrossRef] [PubMed]
    [Google Scholar]
  15. Huch M. , Hanak A. , Specht I. , Dortu C. M. , Thonart P. , Mbugua S. , Holzapfel W. H. , Hertel C. , Franz C. M. . ( 2008; ). Use of Lactobacillus strains to start cassava fermentations for Gari production. . Int J Food Microbiol 128:, 258–267. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kawamura Y. , Hou X.-G. , Sultana F. , Miura H. , Ezaki T. . ( 1995; ). Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus . . Int J Syst Bacteriol 45:, 406–408. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kawamura Y. , Hou X.-G. , Todome Y. , Sultana F. , Hirose K. , Shu S.-E. , Ezaki T. , Ohkuni H. . ( 1998; ). Streptococcus peroris sp. nov. and Streptococcus infantis sp. nov., new members of the Streptococcus mitis group, isolated from human clinical specimens. . Int J Syst Bacteriol 48:, 921–927. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kawamura Y. , Whiley R. A. , Shu S. E. , Ezaki T. , Hardie J. M. . ( 1999; ). Genetic approaches to the identification of the mitis group within the genus Streptococcus . . Microbiology 145:, 2605–2613.[PubMed]
    [Google Scholar]
  19. Kilian M. , Mikkelsen L. , Henrichsen J. . ( 1989; ). Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder 1906). . Int J Syst Bacteriol 39:, 471–484. [CrossRef]
    [Google Scholar]
  20. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kostinek M. , Specht I. , Edward V. A. , Schillinger U. , Hertel C. , Holzapfel W. H. , Franz C. M. . ( 2005; ). Diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of Gari, a traditional African food. . Syst Appl Microbiol 28:, 527–540. [CrossRef] [PubMed]
    [Google Scholar]
  22. Martín V. , Mañes-Lázaro R. , Rodríguez J. M. , Maldonado-Barragán A. . ( 2011; ). Streptococcus lactarius sp. nov., isolated from breast milk of healthy women. . Int J Syst Evol Microbiol 61:, 1048–1052. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Naser, S. M. (2006). In A novel robust identification system of the lactic acid bacteria Enterococcus, Lactobacillus and Streptococcus based on pheS, rpoA and atpA multilocus sequence analysis, pp. 57–79, 267–294. PhD thesis, Ghent University, Ghent, Belgium.
  25. Naser S. , Thompson F. L. , Hoste B. , Gevers D. , Vandemeulebroecke K. , Cleenwerck I. , Thompson C. C. , Vancanneyt M. , Swings J. . ( 2005a; ). Phylogeny and identification of enterococci by atpA gene sequence analysis. . J Clin Microbiol 43:, 2224–2230. [CrossRef] [PubMed]
    [Google Scholar]
  26. Naser S. M. , Thompson F. L. , Hoste B. , Gevers D. , Dawyndt P. , Vancanneyt M. , Swings J. . ( 2005b; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef] [PubMed]
    [Google Scholar]
  27. Pitcher D. G. , Saunders N. A. , Owen R. J. . ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. . Lett Appl Microbiol 8:, 151–156. [CrossRef]
    [Google Scholar]
  28. Poyart C. , Quesne G. , Coulon S. , Berche P. , Trieu-Cuot P. . ( 1998; ). Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. . J Clin Microbiol 36:, 41–47.[PubMed]
    [Google Scholar]
  29. Poyart C. , Quesne G. , Trieu-Cuot P. . ( 2002; ). Taxonomic dissection of the Streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (sodA) sequences: reclassification of ‘Streptococcus infantarius subsp. coli’ as Streptococcus lutetiensis sp. nov. and of Streptococcus bovis biotype 11.2 as Streptococcus pasteurianus sp. nov.. Int J Syst Evol Microbiol 52:, 1247–1255. [CrossRef] [PubMed]
    [Google Scholar]
  30. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35:, 7188–7196. [CrossRef] [PubMed]
    [Google Scholar]
  31. Schloss P. D. , Westcott S. L. , Ryabin T. , Hall J. R. , Hartmann M. , Hollister E. B. , Lesniewski R. A. , Oakley B. B. , Parks D. H. . & other authors ( 2009; ). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. . Appl Environ Microbiol 75:, 7537–7541. [CrossRef] [PubMed]
    [Google Scholar]
  32. Seifert S. , Bub A. , Franz C. M. A. P. , Watzl B. . ( 2011; ). Probiotic Lactobacillus casei Shirota supplementation does not modulate immunity in healthy men with reduced natural killer cell activity. . J Nutr 141:, 978–984. [CrossRef] [PubMed]
    [Google Scholar]
  33. Sistek V. , Maheux A. F. , Boissinot M. , Bernard K. A. , Cantin P. , Cleenwerck I. , De Vos P. , Bergeron M. G. . ( 2012; ). Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 62:, 1314–1320. [CrossRef] [PubMed]
    [Google Scholar]
  34. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. , Kämpfer P. , Maiden M. C. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  35. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  36. Täpp J. , Thollesson M. , Herrmann B. . ( 2003; ). Phylogenetic relationships and genotyping of the genus Streptococcus by sequence determination of the RNase P RNA gene, rnpB . . Int J Syst Evol Microbiol 53:, 1861–1871. [CrossRef] [PubMed]
    [Google Scholar]
  37. Tong H. , Gao X. , Dong X. . ( 2003; ). Streptococcus oligofermentans sp. nov., a novel oral isolate from caries-free humans. . Int J Syst Evol Microbiol 53:, 1101–1104. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  39. Whiley R. A. , Hardie J. M. . ( 2009; ). Genus I. Streptococcus Rosenbach 1884, 22AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 655–711. Edited by De Vos P. , Garrity G. M. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K. H. , Whitman W. B. . . New York:: Springer;.
    [Google Scholar]
  40. Whiley R. A. , Fraser H. Y. , Douglas C. W. I. , Hardie J. M. , Williams A. M. , Collins M. D. . ( 1990; ). Streptococcus parasanguis sp. nov., an atypical viridans Streptococcus from human clinical specimens. . FEMS Microbiol Lett 56:, 115–121.[PubMed] [CrossRef]
    [Google Scholar]
  41. Willcox M. D. P. , Zhu H. , Knox K. W. . ( 2001; ). Streptococcus australis sp. nov., a novel oral streptococcus. . Int J Syst Evol Microbiol 51:, 1277–1281.[PubMed]
    [Google Scholar]
  42. Woo P. C. , Tam D. M. , Leung K. W. , Lau S. K. , Teng J. L. , Wong M. K. , Yuen K. Y. . ( 2002; ). Streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis. . J Clin Microbiol 40:, 805–810. [CrossRef] [PubMed]
    [Google Scholar]
  43. Zbinden A. , Mueller N. J. , Tarr P. E. , Spröer C. , Keller P. M. , Bloemberg G. V. . ( 2012; ). Streptococcus tigurinus sp. nov., isolated from blood of patients with endocarditis, meningitis and spondylodiscitis. . Int J Syst Evol Microbiol 62:, 2941–2945. [CrossRef] [PubMed]
    [Google Scholar]
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