1887

Abstract

A Gram-positive, non-spore-forming bacterium (GW-12028) of unknown origin showing filamentous growth and producing spherical sporangia was studied for its taxonomic allocation. The 16S rRNA gene sequence analysis and subsequent similarity studies showed that strain GW-12028 belongs to the genus , and is most closely related to DSM 43181 (99.9 %) and DSM 43848 (99.6 %) and more distantly related to IFO 14311 (98.4 %) and other species of the genus (95.8 to 98 %). Chemotaxonomic analyses showed that the peptidoglycan diamino acid was -diaminopimelic acid. Whole-cell hydrolysates contained madurose as the diagnostic sugar and exhibited a quinone system that contained predominantly menaquinones with nine isoprenoic units in the side chain [MK-9, MK-9(H), MK-9(H)]. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phoshatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannosides, four unidentified glycolipids, a phospholipid and an aminolipid. The polyamine pattern contained the major compounds spermine and spermidine. The major fatty acids were 10-methyl C, iso-C and C. These chemotaxonomic traits are common to other species of the genus . DNA–DNA hybridizations and physiological and biochemical tests in comparison with the type strains of the most closely related species, and , allowed genotypic and phenotypic differentiation of strain GW-12028. This strain represents a novel species, for which we propose the name sp. nov., with the type strain GW-12028 ( = LMG 27062  = DSM 45763).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.048504-0
2013-07-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/7/2484.html?itemId=/content/journal/ijsem/10.1099/ijs.0.048504-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J.. ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47:, 39–52. [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F.. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef][PubMed]
    [Google Scholar]
  3. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  4. Couch J. N.. ( 1955;). A new genus and family of the Actinomycetales, with a revision of the genus Actinoplanes. . J Elisha Mitchell Sci Soc 71:, 148–155.
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: An approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Iinuma S., Yokota A., Kanamara T.. ( 1996;). New subspecies of the genus Streptosporangium, Streptosporangium amethystogenes subsp. fukuiense subsp. nov. . Actinomycetologica 10:, 35–42. [CrossRef]
    [Google Scholar]
  8. Inahashi Y., Matsumoto A., Ōmura S., Takahashi Y.. ( 2011;). Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins. . J Antibiot (Tokyo) 64:, 297–302. [CrossRef][PubMed]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Steiof M., Dott W.. ( 1991;). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21:, 227–251. [CrossRef]
    [Google Scholar]
  12. Kawamoto I., Takasawa S., Okachi R., Koakura M., Takahashi I., Nara T.. ( 1975;). A new antibiotic victomycin (XK 49-1-B-2). I. Taxonomy and production of the producing organism. . J Antibiot (Tokyo) 28:, 358–365. [CrossRef][PubMed]
    [Google Scholar]
  13. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  14. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  15. Mertz F. P., Yao R. C.. ( 1990;). Streptosporangium carneum sp. nov. isolated from soil. . Int J Syst Bacteriol 40:, 247–253. [CrossRef]
    [Google Scholar]
  16. Nonomura H., Ohara Y.. ( 1960;). Distribution of the actinomycetes in soil. V. The isolation and classification of the genus Streptosporangium. . J Ferment Technol 38:, 405–409.
    [Google Scholar]
  17. Nonomura H., Ohara Y.. ( 1969;). Distribution of the actinomycetes in soil. VII. A culture method effective for both preferential isolation and enumeration of Microbispora and Streptosporangium strains in soil. Part 2. Classification of the isolates. . J Ferment Technol 47:, 701–709.
    [Google Scholar]
  18. Pitcher D. G., Saunders N. A., Owen R. J.. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8:, 151–156. [CrossRef]
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O.. ( 2007;). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. . Nucleic Acids Res 35:, 7188–7196. [CrossRef][PubMed]
    [Google Scholar]
  20. Quintana E. T., Goodfellow M.. ( 2012;). Genus I. Streptosporangium. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol 5B, pp. 1811–1825. Edited by Goodfellow M, Kämpfer P, Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  21. Schäfer D.. ( 1969;). Eine neue Streptosporangium-Art aus türkischer Steppenerde. . Arch Microbiol 66:, 365–373 (in German).
    [Google Scholar]
  22. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  23. Shearer M. C., Colman P. M., Nash C. H. III. ( 1983;). Streptosporangium fragile sp. nov.. Int J Syst Bacteriol 33:, 364–368. [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Kroppenstedt R. M., Jahnke K.-D., Kemmerling C., Gürtler H.. ( 1994;). Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium. . Int J Syst Bacteriol 44:, 265–269. [CrossRef]
    [Google Scholar]
  25. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  26. Stolz A., Busse H.-J., Kämpfer P.. ( 2007;). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:, 572–576. [CrossRef][PubMed]
    [Google Scholar]
  27. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  28. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J.. ( 1983;). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  30. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31:, 241–250. [CrossRef][PubMed]
    [Google Scholar]
  31. Zhang L. P., Jiang C. L., Chen W. X.. ( 2002;). Streptosporangium subroseum sp. nov., an actinomycete with an unusual phospholipid pattern. . Int J Syst Evol Microbiol 52:, 1235–1238. [CrossRef][PubMed]
    [Google Scholar]
  32. Zhang L.-P., Jiang C.-L., Chen W.-X.. ( 2005;). Streptosporangium yunnanense sp. nov. and Streptosporangium purpuratum sp. nov., from soil in China. . Int J Syst Evol Microbiol 55:, 719–724. [CrossRef][PubMed]
    [Google Scholar]
  33. Zhang L.-P., Zhang L.-M., Zhang X.-M.. ( 2009;). Streptosporangium canum sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59:, 1715–1719. [CrossRef][PubMed]
    [Google Scholar]
  34. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R.. ( 1998;). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:, 179–186. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.048504-0
Loading
/content/journal/ijsem/10.1099/ijs.0.048504-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error