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Volume 63, Issue Pt_9

gen. nov., sp. nov., alkalophilic and halotolerant bacteria isolated from soil of the former lake Texcoco (Mexico) Free

Abstract

A novel Gram-positive, rod-shaped, spore-forming bacterium, designated 13CC was isolated from soil of the former lake Texcoco. The strain was aerobic, catalase-positive and oxidase-negative. It grew at salinities of 0–26 % (w/v) NaCl with an optimum at 9–16 % (w/v) NaCl. The cells contain peptidoglycan type A1γ, A1γ′ with glycine instead of -alanine and three variations of peptidoglycan type A4γ. The only quinone detected was MK-7. The major fatty acid was anteiso-C. The polar lipids fraction consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different phospholipids. The DNA GC content was 37.5 mol%. Maximum-likelihood phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 13CC was closely related to members of the genus and shared 92.35 % similarity with , 92.28 % with and 92.21 % with . It is proposed based on the phenotypic, genotypic and phylogenetic analyses that the novel isolate should be classified as a representative of a new genus and novel species, for which the name gen. nov., sp. nov. is proposed. The type strain of is 13CC ( = JCM 17654 = DSM 24696).

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Funding
This study was supported by the:
  • Instituto de Ciencia y Tecnología del Distrito Federal (Award ICyTDF/295/2009)
  • Consejo Nacional de Ciencia y Tecnología (CONACYT)
© 2013 IUMS
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2013-09-01
2024-04-25
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Texcoconibacillus texcoconensis gen. nov., sp. nov., alkalophilic and halotolerant bacteria isolated from soil of the former lake Texcoco (Mexico)
Int J Syst Evol Microbiol 63, 3336 (2013); https://doi.org/10.1099/ijs.0.048447-0
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