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Abstract
A Gram-reaction-negative, non-spore-forming, gliding, non-translucent, colourless or yellow, aerobic and elevated-colony-forming strain, designated E403T, was isolated from the Bashi Channel and subjected to a polyphasic taxonomic study. Strain E403T could grow in the presence of 0.3–8 % (w/v) NaCl, at 16–43 °C and at pH 6–9, and grew optimally at 28 °C, pH 8, in natural seawater medium. The respiratory quinones were MK-6 and MK-7. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), iso-C15 : 0 3-OH and C16 : 0. The DNA G+C content of strain E403T was 37.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences of members of the family Flavobacteriaceae showed that strain E403T formed a distinct evolutionary lineage within the stable cluster containing type strains Zhouia amylolytica HN-171T (92.2 % similarity) and Joostella marina En5T (92.4 % similarity). In addition to the large 16S rRNA gene sequence differences, E403T can also be distinguished from the reference type strains J. marina En5T and Sinomicrobium oceani SCSIO 03483T by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain E403T is suggested to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Pustulibacterium marinum gen. nov., sp. nov. is proposed. The type strain is E403T ( = CCTCC AB2012862T = CGMCC 1.12333T = KCTC 32192T).
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Funding
- National Basic Research Program of China (Award 2010CB833801)
- National Natural Science Foundation of China (Award 41206136)
- Guangdong Province and Chinese Academy of Science cooperation Foundation (Award 2012B091100276)
- Public Science and Technology Research Funds Projects of Ocean (Award 201005031-5)
- Provincial Collaborative Foundation Project of Guangdong (Award 9351007002000001)
- Key Laboratory of Marine Bio-resources Sustainable Utilization, Chinese Academy of Science