1887

Abstract

A Gram-stain-negative, rod-shaped bacterium with appendages, designated Ar-22, was isolated from a seawater sample collected from the western part of Prydz Bay, near Cape Darnley, Antarctica. Strain Ar-22 grew optimally at 35 °C, at pH 7.5 and in the presence of 1–3 % (w/v) NaCl. The isolate was positive for casein, gelatin and Tween 20 decomposition and negative for HS production and indole formation. Chemotaxonomic analysis showed that MK-6 was the major isoprenoid quinone and phosphatidylethanolamine was the major polar lipid. The major fatty acids were iso-C 3-OH, iso-C G, iso-C and Cω7/iso-C 2OH. The genomic DNA G+C content was 44.8 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain Ar-22 is closely related to members of the genus , sharing 94.2–97.3 % sequence similarity with the type strains of species of the genus and being most closely related to the . Phylogenetic analysis based on the 16S rRNA gene sequence comparison confirmed that strain Ar-22 formed a deep lineage with Sequence similarity between strain Ar-22 and DSM 13258, the type species of the genus , was 96.9 %. Strain Ar-22 exhibited mean DNA–DNA relatedness values of 40.1 %, 49.4 % and 25.7 % to JCM 11811, JCM 11812 and KCTC 22173, respectively. On the basis of phenotypic and genotypic data, strain Ar-22 represents a novel species of the genus for which the name sp. nov. (type strain Ar-22 = CGMCC 1.12174 = JCM 18450) is proposed.

Funding
This study was supported by the:
  • , Polar Science Strategic Research Foundation of China
  • , Key Laboratory Open Foundation of Polar Science, State Oceanic Administration , (Award 20100216)
  • , Public Science and Technology Research Funds Projects of Ocean , (Award 201005032-3)
  • , Scientific Research Fund of the Second Institute of Oceanography, State Oceanic Administration , (Award JT1011)
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2013-09-01
2020-09-26
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