1887

Abstract

has been used as a medicinal plant for thousands of years in Mexico. Rhizobial strains were obtained from root nodules of collected from different geographical regions in Chiapas and characterized by BOX-PCR, amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequence analysis. Most isolates corresponded to members of the genus and those not related to species with validly published names were further characterized by , , and gene phylogenies, phenotypic and DNA–DNA hybridization analyses. Three novel related species of the genus within the ‘ group’ share the same symbiovar that may be named sv. . The names proposed for the three novel species are sp. nov. (type strain, CCGE524 = ATCC BAA-2435 = CIP 110456 = LBP2-1), sp. nov. (type strain, CCGE526 = ATCC BAA-2446 = CIP 110454 = NSJP1-1) and sp. nov. (type strain, CCGE525 = ATCC BAA-2445 = CIP 110453 = SJP1-2).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.048249-0
2013-09-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3423.html?itemId=/content/journal/ijsem/10.1099/ijs.0.048249-0&mimeType=html&fmt=ahah

References

  1. De Baere T., de Mendonca R., Claeys G., Verschraegen G., Mijs W., Verhelst R., Rottiers S., Van Simaey L., De Ganck C., Vaneechoutte M.. ( 2002;). Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory. . BMC Microbiol 2:, 4. [CrossRef][PubMed]
    [Google Scholar]
  2. de Lajudie P., Willems A., Nick G., Moreira F., Molouba F., Hoste B., Torck U., Neyra M., Collins M. D.. & other authors ( 1998;). Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov.. Int J Syst Bacteriol 48:, 369–382. [CrossRef][PubMed]
    [Google Scholar]
  3. Delajudie P., Willems A., Pot B., Dewettinck D., Maestrojuan G., Neyra M., Collins M. D., Dreyfus B., Kersters K., Gillis M.. ( 1994;). Polyphasic taxonomy of rhizobia: Emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov, Sinorhizobium saheli sp. nov, and Sinorhizobium teranga sp. nov.. Int J Syst Bacteriol 44:, 715–733. [CrossRef]
    [Google Scholar]
  4. Díaz J. L.. ( 1976;). Algunas plantas mexicanas con efectos sobre el sistema nervioso. . In Estado Actual del Conocimiento en Plantas Medicinales Mexicanas, pp. 109–130. Edited by Losoya X... México:: Instituto Mexicano para el Estudio de las Plantas Medicinales (IMEPLAM); (in Spanish)
  5. Eardly B. D., Wang F. S., Whittam T. S., Selander R. K.. ( 1995;). Species limits in Rhizobium populations that nodulate the common bean (Phaseolus vulgaris). . Appl Environ Microbiol 61:, 507–512.[PubMed]
    [Google Scholar]
  6. Edgar R. C.. ( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32:, 1792–1797. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Gaunt M. W., Turner S. L., Rigottier-Gois L., Lloyd-Macgilp S. A., Young J. P.. ( 2001;). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. . Int J Syst Evol Microbiol 51:, 2037–2048. [CrossRef][PubMed]
    [Google Scholar]
  9. Geniaux E., Flores M., Palacios R., Martínez E.. ( 1995;). Presence of megaplasmids in Rhizobium tropici and further evidence of differences between the two R. tropici subtypes. . Int J Syst Bacteriol 45:, 392–394. [CrossRef]
    [Google Scholar]
  10. Hernandez-Lucas I., Segovia L., Martinez-Romero E., Pueppke S. G.. ( 1995;). Phylogenetic relationships and host range of Rhizobium spp. that nodulate Phaseolus vulgaris L.. Appl Environ Microbiol 61:, 2775–2779.[PubMed]
    [Google Scholar]
  11. Hynes M. F., McGregor N. F.. ( 1990;). Two plasmids other than the nodulation plasmid are necessary for formation of nitrogen-fixing nodules by Rhizobium leguminosarum. . Mol Microbiol 4:, 567–574. [CrossRef][PubMed]
    [Google Scholar]
  12. Khamis A., Colson P., Raoult D., Scola B. L.. ( 2003;). Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species, including a strategy for choosing discriminative partial sequences. . Appl Environ Microbiol 69:, 6740–6749. [CrossRef][PubMed]
    [Google Scholar]
  13. Konstantinidis K. T., Ramette A., Tiedje J. M.. ( 2006;). Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. . Appl Environ Microbiol 72:, 7286–7293. [CrossRef][PubMed]
    [Google Scholar]
  14. Lloret L., Ormeño-Orrillo E., Rincón R., Martínez-Romero J., Rogel-Hernández M. A., Martínez-Romero E.. ( 2007;). Ensifer mexicanus sp. nov. a new species nodulating Acacia angustissima (Mill.) Kuntze in Mexico. . Syst Appl Microbiol 30:, 280–290. [CrossRef][PubMed]
    [Google Scholar]
  15. Martínez-Romero E., Segovia L., Mercante F. M., Franco A. A., Graham P., Pardo M. A.. ( 1991;). Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. . Int J Syst Bacteriol 41:, 417–426. [CrossRef][PubMed]
    [Google Scholar]
  16. Martínez-Romero J. C., Ormeño-Orrillo E., Rogel-Hernández M. A., López-López A., Martínez-Romero E.. ( 2010;). Trends in rhizobial evolution and some taxonomic remarks. . In: Evolutionary Biology – Concepts, Molecular and Morphological Evolution. pp. 301–315. Edited by Pontarotti P... Berlin:: Springer-Verlag;. [CrossRef]
    [Google Scholar]
  17. Meckes M., Villarreal M. L., Tortoriello J., Berlin B., Berlin E. A.. ( 1995;). A microbiological evaluation of medicinal-plants used by the Maya people of Southern Mexico. . Phytother Res 9:, 244–250. [CrossRef]
    [Google Scholar]
  18. Nimnoi P., Lumyong S., Pongsilp N.. ( 2011;). Impact of rhizobial inoculants on rhizosphere bacterial communities of three medicinal legumes assessed by denaturing gradient gel electrophoresis (DGGE). . Ann Microbiol 61:, 237–245. [CrossRef]
    [Google Scholar]
  19. Pandey P., Sahgal M., Maheswari D. K., Johri B. N.. ( 2004;). Genetic diversity of rhizobia isolated from medicinal legumes growing in the sub-Himalayan region of Uttaranchal. . Curr Sci 86:, 202–207.
    [Google Scholar]
  20. Ribeiro R. A., Rogel M. A., López-López A., Ormeño-Orrillo E., Gomes Barcellos F., Martínez J., Lopes Thompson F., Martínez-Romero E., Hungria M.. ( 2011;). Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov. . Int J Syst Evol Microbiol 62:, 1179–1184. [CrossRef][PubMed]
    [Google Scholar]
  21. Rincón-Rosales R., Lloret L., Ponce E., Martínez-Romero E.. ( 2009;). Rhizobia with different symbiotic efficiencies nodulate Acaciella angustissima in Mexico, including Sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with Sinorhizobium mexicanum. . FEMS Microbiol Ecol 67:, 103–117. [CrossRef][PubMed]
    [Google Scholar]
  22. Rogel M. A., Ormeño-Orrillo E., Martínez Romero E.. ( 2011;). Symbiovars in rhizobia reflect bacterial adaptation to legumes. . Syst Appl Microbiol 34:, 96–104. [CrossRef][PubMed]
    [Google Scholar]
  23. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  25. Tamura K., Nei M.. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol 10:, 512–526.[PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  27. Toledo I., Lloret L., Martínez-Romero E.. ( 2003;). Sinorhizobium americanus sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico. . Syst Appl Microbiol 26:, 54–64. [CrossRef][PubMed]
    [Google Scholar]
  28. Valverde A., Igual J. M., Peix A., Cervantes E., Velázquez E.. ( 2006;). Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris. . Int J Syst Evol Microbiol 56:, 2631–2637. [CrossRef][PubMed]
    [Google Scholar]
  29. Versalovic J., Schneider M., De Bruijn F. J., Lupski J. R.. ( 1994;). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. . Methods Mol Cell Biol 5:, 25–40.
    [Google Scholar]
  30. Vincent J. M.. ( 1970;). A Manual for the Practical Study of Root Nodule Bacteria. Oxford:: Blackwell Scientific Publications;.
    [Google Scholar]
  31. Vinuesa P., Silva C., Lorite M. J., Izaguirre-Mayoral M. L., Bedmar E. J., Martínez-Romero E.. ( 2005;). Molecular systematics of rhizobia based on maximum likelihood and Bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania microsymbionts from Venezuelan wetlands. . Syst Appl Microbiol 28:, 702–716. [CrossRef][PubMed]
    [Google Scholar]
  32. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.048249-0
Loading
/content/journal/ijsem/10.1099/ijs.0.048249-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error