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Volume 63, Issue Pt_11

Description of gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify and as comb. nov., comb. nov., comb. nov. and comb. nov. based on polyphasic analysis Free

Abstract

An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3, was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3 grew at 20–40 °C, pH 5.0–10.0 and <2 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3 showed the greatest degree of similarity to SUEMI10 (96.5 %), SUEMI08 (96.3 %), SUEMI03 (96.0 %), PB1 (95.4 %) and members of other species (94.1–95.2 %), and lower similarity to members of other genera (<94 %). Phylogenetic analyses also positioned the novel strain in the genus as an independent lineage. The major fatty acids in strain CC-AFH3 were C 3-OH, C, C 2-OH, C, iso-C 3-OH, C cyclo, Cω7/Cω6 and Cω7/Cω6. The major polar lipids of strain CC-AFH3 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3 represents a novel species in a new genus, for which we propose the name gen. nov., sp. nov.; the type strain of is CC-AFH3 ( = BCRC 80516 = JCM 18414). We also propose the reclassification of , , and ‘’ as comb. nov. (type strain SUEMI10 = LMG 26149 = CECT 7840), comb. nov. (type strain SUEMI08 = LMG 26150 = CECT 7839), comb. nov. (type strain SUEMI03 = LMG 26151 = CECT 7838) and comb. nov. (type strain PB1 = DSM 26001 = LMG 27282), respectively. An emended description of is also presented.

  • Published Online:
Funding
This study was supported by the:
  • National Science Council
  • Council of Agriculture
  • Executive Yuan
  • Ministry of Education, Taiwan, ROC
© 2013 IUMS
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2013-11-01
2024-04-23
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Description of Noviherbaspirillum malthae gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify Herbaspirillum soli,Herbaspirillum aurantiacum,Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspirillum soli comb. nov., Noviherbaspirillum aurantiacum comb. nov., Noviherbaspirillum canariense comb. nov. and Noviherbaspirillum psychrotolerans comb. nov. based on polyphasic analysis
Int J Syst Evol Microbiol 63, 4100 (2013); https://doi.org/10.1099/ijs.0.048231-0
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