1887

Abstract

An aerobic, asporous, flagellated, Gram-stain-positive, rod-shaped bacterium MD-T1-10-2 was isolated from the topsoil of Midui Glacier, Tibet Province, China. Phylogenetic analysis based on 16S rRNA gene sequence analysis placed the strain in a clade containing CCTCC AB 209002, DSM 18580, JCM 11656, JCM 11654 and JCM 11655, with the sequence similarities of 99.2, 98.1, 96.7, 96.6 and 96.4 %, respectively. DNA–DNA hybridization analysis indicated that strain MD-T1-10-2 represented a new member of this genus. The optimal ranges of temperature and pH for growth were 20–25 °C and 7.0–9.0, respectively; the strain could even grow at 0 °C. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The predominant menaquinones were MK-10 and MK-11. The cell wall amino acids were lysine, alanine, glycine and glutamic acids. The DNA G+C content was 65.9 mol%. Based on the genotypic and phenotypic data, strain MD-T1-10-2 for which the name sp. nov. is proposed; the type strain is MD-T1-10-2 ( = CGMCC 1.11101 = NBRC 107877).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31070001)
  • Knowledge Innovation Program of Chinese Academy of Sciences (Award KSCX2-YW-Z-0937)
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2013-07-01
2021-10-26
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