@article{mbs:/content/journal/ijsem/10.1099/ijs.0.047951-0, author = "Kim, Duwoon and Baik, Keun Sik and Hwang, Ye Seul and Choi, Jong-Soon and Kwon, Joseph and Seong, Chi Nam", title = "Vibriohemicentroti sp. nov., an alginate lyase-producing bacterium, isolated from the gut microflora of sea urchin (Hemicentrotus pulcherrimus)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_10", pages = "3697-3703", doi = "https://doi.org/10.1099/ijs.0.047951-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.047951-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "An alginate lyase-producing bacterium, designated AlyHP32T, was isolated from the gut of sea urchin (Hemicentrotus pulcherrimus) obtained from the South Sea, Republic of Korea. Cells of strain AlyHP32T were Gram-reaction-negative and motile with a single polar flagellum. The strain grew with 1–6 % (w/v) NaCl (optimum 2–4 %) and at 4–30 °C (optimum 15–25 °C). Phylogenetic analysis based on sequences of the 16S rRNA gene and five housekeeping genes (atpA, pyrH, recA, rpoA and rpoD) revealed that strain AlyHP32T belonged to the genus Vibrio and formed a compact clade with the Vibrio splendidus group. However, DNA–DNA hybridization and fingerprints using the repetitive primers BOX and REP indicated that strain AlyHP32T was distinct from closely related species of the genus Vibrio . The major fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0. The DNA G+C content was 44.1 mol%. The predominant quinone was ubiquinone Q-8. Based on genotypic, phenotypic and DNA–DNA hybridization analysis, strain AlyHP32T represents a novel species of the genus Vibrio ; the name Vibrio hemicentroti sp. nov. (type strain AlyHP32T = KCTC 32085T = DSM 26178T) is proposed for this novel taxon.", }