1887

Abstract

An alginate lyase-producing bacterium, designated AlyHP32, was isolated from the gut of sea urchin () obtained from the South Sea, Republic of Korea. Cells of strain AlyHP32 were Gram-reaction-negative and motile with a single polar flagellum. The strain grew with 1–6 % (w/v) NaCl (optimum 2–4 %) and at 4–30 °C (optimum 15–25 °C). Phylogenetic analysis based on sequences of the 16S rRNA gene and five housekeeping genes (, , , and ) revealed that strain AlyHP32 belonged to the genus and formed a compact clade with the group. However, DNA–DNA hybridization and fingerprints using the repetitive primers BOX and REP indicated that strain AlyHP32 was distinct from closely related species of the genus . The major fatty acids were summed feature 3 (Cω7 and/or Cω6) and C. The DNA G+C content was 44.1 mol%. The predominant quinone was ubiquinone Q-8. Based on genotypic, phenotypic and DNA–DNA hybridization analysis, strain AlyHP32 represents a novel species of the genus ; the name sp. nov. (type strain AlyHP32 = KCTC 32085 = DSM 26178) is proposed for this novel taxon.

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2013-10-01
2020-01-19
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