1887

Abstract

A novel actinomycete, strain 44EHW, was isolated from rhizospheric soil under an Elephant ear plant () in Bangkok, Thailand. Strain 44EHW produced long branching hyphae and abundant aerial mycelia with chains of rod-shaped spores. Whole-cell hydrolysates contained galactose, glucose, arabinose, ribose, mannose and rhamnose as diagnostic sugars. -Diaminopimelic acid was the diamino acid and glycine, alanine and glutamic acid were present in the cell-wall peptidoglycan with the acyl type of the peptidoglycan being acetyl. Phospholipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids and diphosphatidylglycerol, as well as other unknown phospholipids; however, no mycolic acids were detected. The predominant menaquinone observed was MK-9(H) and major fatty acids were iso-C and 2-OH iso-C. The G+C content of genomic DNA was 74 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate was most similar to NBRC 16517. However, DNA–DNA hybridization revealed a low relatedness between this isolate and NBRC 16517, indicating that this isolate represented a novel species in the genus . On the basis of 16S rRNA gene sequence analysis, phenotypic characteristics and DNA–DNA hybridization data, we propose that strain 44EHW represents a novel species in the genus . The type strain is 44EHW ( = BCC 53155 = NBRC 108932).

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2013-07-01
2019-10-22
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References

  1. Becker B. , Lechevalier M. P. , Lechevalier H. A. . ( 1965; ). Chemical composition of cell-wall preparation from strains of various form-genera of aerobic actinomycetes. . Appl Microbiol 13:, 236–243.[PubMed]
    [Google Scholar]
  2. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a species tree topology. . Syst Biol 20:, 406–416. [CrossRef]
    [Google Scholar]
  5. Hasegawa T. . ( 1988; ). Actinokineospora: a new genus of the Actinomycetales. . Actinomycetologica 2:, 31–45. [CrossRef]
    [Google Scholar]
  6. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  7. Hayakawa M. , Sadakata T. , Kajiura T. , Nonomura H. . ( 1991; ). New methods for the highly selective isolation of Micromonospora and Microbispora from soil. . J Ferment Bioeng 72:, 320–326. [CrossRef]
    [Google Scholar]
  8. Jacobson E. , Grauville W. C. , Fogs C. E. . ( 1958; ). Color Harmony Manual, , 4th edn.. Chicago:: Container Corporation of America;.
    [Google Scholar]
  9. Kawamoto I. , Oka T. , Nara T. . ( 1981; ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146:, 527–534.[PubMed]
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Labeda D. P. , Price N. P. , Tan G. Y. A. , Goodfellow M. , Klenk H.-P. . ( 2010; ). Emended description of the genus Actinokineospora Hasegawa 1988 and transfer of Amycolatopsis fastidiosa Henssen et al. 1987 as Actinokineospora fastidiosa comb. nov.. Int J Syst Evol Microbiol 60:, 1444–1449. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lisdiyanti P. , Otoguro M. , Ratnakomala S. , Lestari Y. , Hastuti R. D. , Triana E. , Katsuhiko A. , Widyastuti Y. . ( 2010; ). Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter. . Int J Syst Evol Microbiol 60:, 2331–2335. [CrossRef] [PubMed]
    [Google Scholar]
  13. Matsumoto A. , Yamada M. , ōmura S. , Takahashi Y. . ( 2008; ). Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae. . Int J Syst Evol Microbiol 58:, 1019–1023. [CrossRef] [PubMed]
    [Google Scholar]
  14. Minnikin D. E. , Patel P. V. , Alshamaony L. , Goodfellow M. . ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  15. Otoguro M. , Hayakawa M. , Yamazaki T. , Tamura T. , Hatano K. , Iimura Y. . ( 2001; ). Numerical phenetic and phylogenetic analyses of Actinokineospora isolates, with a description of Actinokineospora auranticolor sp. nov. and Actinokineospora enzanensis sp. nov.. Actinomycetol 15:, 30–39. [CrossRef]
    [Google Scholar]
  16. Rzhetsky A. , Nei M. . ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  17. Saito H. , Miura K. I. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  20. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  21. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  22. Tamura T. , Hayakawa M. , Nonomura H. , Yokota A. , Hatano K. . ( 1995; ). Four new species of the genus Actinokineospora: Actinokineospora inagensis sp. nov., Actinokineospora globicatena sp. nov., Actinokineospora terrae sp. nov., and Actinokineospora diospyrosa sp. nov. . Int J Syst Bacteriol 45:, 371–378. [CrossRef]
    [Google Scholar]
  23. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tang X. , Zhou Y. , Zhang J. , Ming H. , Nie G. , Yang L. , Tang S. , Li W. . ( 2012; ). Actinokineospora soli sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Actinokineospora . . Int J Syst Evol Microbiol 62:, 1845–1849. [CrossRef] [PubMed]
    [Google Scholar]
  25. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tomiyasu I. . ( 1982; ). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . . J Bacteriol 151:, 828–837.[PubMed]
    [Google Scholar]
  27. Uchida K. , Aida K. . ( 1977; ). Acyl type of bacterial cell wall: its simple identification by a colorimetric method. . J Gen Appl Microbiol 23:, 249–260. [CrossRef]
    [Google Scholar]
  28. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. , Stackebrandt E. , Srarr M. P. , Trüper H. G. . ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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