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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of gen. nov., sp. nov., belonging to the family Free

Abstract

Cauliform bacteria are prosthecate bacteria often specialized for oligotrophic environments. A polyphasic approach, comprising 16S rRNA gene sequencing, lipid analysis and salt tolerance characterizations, was used to clarify the taxonomy of one isolate, strain MCS 33, obtained from above the hot water plume of a deep-sea hydrothermal vent near Vancouver island, Canada. Cells contained no detectable phospholipids or sulpholipids, but did contain 1,2-di-O-acyl-3-O-α--glucopyranosylglycerol, 1,2-di-O-acyl-3-O-α--glucopyranuronosylglycerol and the novel lipid 1,2-di-O-acyl-3-[O-α--glucopyranuronosyl]glycerol-6′--glycine. It is assumed that the various glucoronosyl lipids are replacing, at least partially, the phospholipids in their various tasks in the cell cycle. The GC content of the genomic DNA of strain MCS 33 was 62.8 mol%, and Q10 was the predominant respiratory ubiquinone. The 16S rRNA gene sequence of this chemoheterotrophic, aerobic, moderately halophilic strain showed only a low similarity of 94.4 % to that of C116-18, and both strains also differed based on their lipids. Although the novel strain was isolated from seawater sampled near a hydrothermal vent, its optimum temperature for growth was 30 °C. The main cellular fatty acids were Cω7, C and the unknown fatty acid ECL 11.798, and the main hydroxy fatty acid was C 3-OH. The strain is proposed to represent a novel species of a new genus, gen. nov., sp. nov. The type strain of the type species is MCS 33 ( = LMG 27140 = CCUG 62981).

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Funding
This study was supported by the:
  • German Federal Ministry for Science, Education and Research (Award 0319433C)
  • European Union
© 2013 IUMS
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2013-06-01
2024-04-19
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Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae
Int J Syst Evol Microbiol 63, 2207 (2013); https://doi.org/10.1099/ijs.0.047894-0
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