A spore-forming streptomycete designated strain SUK12T was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12T in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340T (98.2 %) followed by Streptomyces chrestomyceticus NRRL B-3310T (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LL-diaminopimelic acid as a component of cell-wall peptidoglycan; C12 : 0, C14 : 0, C15 : 0 and C17 : 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12T to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12T from the related species of the genus Streptomyces. The DNA–DNA relatedness value between strain SUK12T and S. corchorusii DSM 40340T is 18.85±4.55 %. Strain SUK12T produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12T ( = DSM 42048T = NRRL B-24860T) be classified in the genus Streptomyces as the type strain of Streptomyceskebangsaanensis sp. nov.
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Funding
This study was supported by the:
Universiti Kebangsaan Malaysia
(Award UKM-GUP-TKP-08-22-074)
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res25, 3389–3402. [View Article][PubMed]
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.(1977). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem81, 461–466. [View Article][PubMed]
CoombsJ. T.,
FrancoC. M. M.(2003). Isolation and identification of actinobacteria from surface-sterilized wheat roots. . Appl Environ Microbiol69, 5603–5608. [View Article][PubMed]
De LeyJ.,
CattoirH.,
ReynaertsA.(1970). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12, 133–142. [View Article][PubMed]
EuzébyJ. P.(2012). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . (Last full update: 3 June 2012). http://www.bacterio.cict.fr/.
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24, 54–63. [View Article]
HussV. A. R.,
FestlH.,
SchleiferK. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol4, 184–192. [View Article][PubMed]
KämpferP.(2006). The family Streptomycetaceae, part I: taxonomy. . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 3, pp. 538–604. Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK. H.,
StackebrandtE.
. New York:: Springer;.
KellyK. L.(1964).Inter-Society Color Council-National Bureau of Standards 246 Color-Name Charts Illustrated With Centroid Colors. Washington, DC:: US Government Print Office;.
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
Korn-WendishF.,
KutznerH. J.(1992). The family Streptomycetaceae
. . In The Prokaryotes, pp. 921–995. Edited by
BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK. H.
. New York:: Springer;.
MandelM.,
MarmurJ.(1968). The use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol12B, 195–206. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettI. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
MordarskaH.,
MordarskiM.,
GoodfellowM.(1972). Chemotaxonomic characters and classification of some nocardioform bacteria. . J Gen Microbiol71, 77–86. [View Article][PubMed]
OwenR. J.,
PitcherD.(1985). Current methods for estimating DNA base composition and levels of DNA–DNA hybridization. . In Chemical Methods in Bacterial Systematics, pp. 67–93. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
ShirlingE. B.,
GottliebD.(1969). Cooperative description of type cultures of Streptomyces. IV. Species description from second, third and fourth studies. . Int J Syst Bacteriol19, 391–512. [View Article]
ShirlingE. B.,
GottliebD.(1972). Cooperative description of type strains of Streptomyces. V. Additional descriptions. . Int J Syst Bacteriol22, 265–394. [View Article]
TresnerH. D.,
HayesJ. A.,
BackusE. J.(1968). Differential tolerance of streptomycetes to sodium chloride as a taxonomic aid. . Appl Microbiol16, 1134–1136.[PubMed]