sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid Free

Abstract

A spore-forming streptomycete designated strain SUK12 was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus . Morphological and chemical characteristics of the strain were consistent with those of members of the genus . Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12 in the genus where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to DSM 40340 (98.2 %) followed by NRRL B-3310 (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H) as the major menaquinone; -diaminopimelic acid as a component of cell-wall peptidoglycan; C, C, C and C as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12 to the genus . The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12 from the related species of the genus . The DNA–DNA relatedness value between strain SUK12 and DSM 40340 is 18.85±4.55 %. Strain SUK12 produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12 ( = DSM 42048 = NRRL B-24860) be classified in the genus as the type strain of sp. nov.

Funding
This study was supported by the:
  • Universiti Kebangsaan Malaysia (Award UKM-GUP-TKP-08-22-074)
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2013-10-01
2024-03-28
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