1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

sp. nov., a moderately halophilic bacterium isolated from the surface of a deep-sea fish, , and emended description of the genus Free

Abstract

A moderately halophilic, slightly acidophilic, aerobic bacterium, designated strain YTM-1, was isolated from the body surface of . Cells were Gram-stain-negative, short rods or cocci, approximately 0.9–1.1 µm long and 1.0–1.8 µm wide. Strain YTM-1 was able to grow with 1–30 % NaCl (optimum, 7.5–10 %, w/v), at 4–30 °C (optimum, 20–25 °C) and at pH 3.8–9.5 (optimum, pH 5.0–5.5). Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain YTM-1 belonged to the genus with low similarity values to the type strains of recognized species of this genus (<94.8–94.4 %). The polar lipids of strain YTM-1 consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, three unknown phospholipids and one unknown lipid. The predominant isoprenoid quinone was Q-8. The major fatty acids were Cω8 cyclo, Cω7, Cω5 and C. The DNA G+C content of strain YTM-1 was 67.3 mol%. These phylogenetic, physiological and chemotaxonomic data indicated that strain YTM-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YTM-1 ( = JCM 18087 = CECT 8012). An emended description of the genus is also proposed.

  • Published Online:
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047845-0
2013-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/2180.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047845-0&mimeType=html&fmt=ahah

References

  1. Antunes A., Eder W., Fareleira P., Santos H., Huber R. ( 2003 ). Salinisphaera shabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine–seawater interface of the Shaban Deep, Red Sea. . Extremophiles 7, 29–34.[PubMed]
    [Google Scholar]
  2. Bae G. D., Hwang C. Y., Kim H. M., Cho B. C. ( 2010 ). Salinisphaera dokdonensis sp. nov., isolated from surface seawater. . Int J Syst Evol Microbiol 60, 680–685. [View Article] [PubMed]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  4. Crespo-Medina M., Chatziefthimiou A., Cruz-Matos R., Pérez-Rodríguez I., Barkay T., Lutz R. A., Starovoytov V., Vetriani C. ( 2009 ). Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultative autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera . . Int J Syst Evol Microbiol 59, 1497–1503. [View Article] [PubMed]
    [Google Scholar]
  5. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484–485.[PubMed]
    [Google Scholar]
  6. Echigo A., Minegishi H., Shimane Y., Kamekura M., Usami R. ( 2012 ). Natribacillus halophilus gen. nov., sp. nov., a moderately halophilic and alkalitolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 62, 289–294. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  8. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  9. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 2947–2948. [View Article] [PubMed]
    [Google Scholar]
  10. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for extracting bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  11. Nelson J. S. ( 2006 ). Fishes of the World, , 4th edn.. Hoboken, NJ:: Wiley;.
     [Google Scholar]
  12. Park S. J., Cha I. T., Kim S. J., Shin K. S., Hong Y., Roh D. H., Rhee S. K. ( 2012 ). Salinisphaera orenii sp. nov., isolated from a solar saltern. . Int J Syst Evol Microbiol 62, 1877–1883. [View Article] [PubMed]
    [Google Scholar]
  13. Saito H., Miura K. I. ( 1963 ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72, 619–629. [View Article] [PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  15. Shimane Y., Hatada Y., Minegishi H., Echigo A., Nagaoka S., Miyazaki M., Ohta Y., Maruyama T., Usami R. et al. ( 2011 ). Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt. . Int J Syst Evol Microbiol 61, 2266–2270. [View Article] [PubMed]
    [Google Scholar]
  16. Shinohara G., Yabe M., Amaoka K., Meguro T. ( 1992 ). A psychrolutid, Malacocottus gibber, collected from the mesopelagic zone of the Sea of Japan, with comments on its intraspecific variation. . Jpn J Ichthyol 38, 419–424.
     [Google Scholar]
  17. Smibert R. M., Krieg N. R. ( 1981 ). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  18. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  19. Stamatakis A., Ludwig T., Meier H. ( 2005 ). RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. . Bioinformatics 21, 456–463. [View Article] [PubMed]
    [Google Scholar]
  20. Tamaoka J. ( 1986 ). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. . Methods Enzymol 123, 251–256. [View Article] [PubMed]
    [Google Scholar]
  21. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  22. Tsuruwaka Y., Shimada E. ( 2011 ). Rearing and spawning of the deep-sea fish Malacocottus gibber in the laboratory. . Ichthyol Res 58, 188–190. [View Article]
    [Google Scholar]
  23. Widdel F., Kohring G. W., Mayer F. ( 1983 ). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. . Arch Microbiol 134, 286–294. [View Article]
    [Google Scholar]
  24. Zhang Y. J., Tang S. K., Shi R., Klenk H. P., Chen C., Yang L. L., Zhou Y., Li W. J. ( 2012 ). Salinisphaera halophila sp. nov., a moderately halophilic bacterium isolated from brine of a salt well. . Int J Syst Evol Microbiol 62, 2174–2179. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047845-0
Loading
Salinisphaera japonica sp. nov., a moderately halophilic bacterium isolated from the surface of a deep-sea fish, Malacocottus gibber, and emended description of the genus Salinisphaera
Int J Syst Evol Microbiol 63, 2180 (2013); https://doi.org/10.1099/ijs.0.047845-0
/content/journal/ijsem/10.1099/ijs.0.047845-0
/content/journal/ijsem/10.1099/ijs.0.047845-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error