A novel Gram-negative, non-spore-forming, rod-shaped strain, H1T, was isolated from activated sludge by micromanipulation. No close relatives among cultured bacterial isolates were found; phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H1T forms a deep single branch in the family Rhodospirillaceae. Cells of strain H1T were slightly curved to straight rods (1.2–1.4×1.5–1.7 µm) and motile by a single polar flagellum. Strain H1T was able to grow in the presence of 0–4 % NaCl and grew optimally at 37 °C and pH 6.0–7.0. Chemotaxonomic analysis revealed that strain H1T possessed Q-10 as the predominant ubiquinone and C18 : 1ω7c, C16 : 0 and C19 : 0 cyclo ω8c as the major fatty acids. The DNA G+C content of strain H1T was 65.1 mol%. Comparative analysis of 16S rRNA gene sequences, and phenotypic and chemotaxonomic data, indicate that strain H1T should represent a novel genus and species of the family Rhodospirillaceae. The name Taonella mepensis gen. nov., sp. nov. is proposed. The type strain of Taonella mepensis is H1T ( = CICC 10529T = CCTCC AB 2012861T = KACC 16940T).
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Funding
This study was supported by the:
Fund for Distinguished Young Scientist of Jiangsu Province
(Award BK2012029)
National Key Technology R&D Program
(Award 2012BAD14B02)
‘948’ project of Ministry of Agriculture
(Award 2011-Z21)
Fundamental Research Funds for the Central Universities
(Award KYZ200920)
ImhoffJ. F.,
PetriR.,
SülingJ.(1998). Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov, and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov.. Int J Syst Bacteriol48, 793–798. [View Article] [PubMed]
KawasakiH.,
HoshinoY.,
KuraishiH.,
YamasatoK.(1992).Rhodocista centenaria gen. nov, sp. nov., a cyst-forming anoxygenic photosynthetic bacterium and its phylogenetic position in the Proteobacteria alpha-group. . J Gen Appl Microbiol38, 541–551. [View Article]
LaiQ.,
YuanJ.,
WuC.,
ShaoZ.(2009).Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. . Int J Syst Evol Microbiol59, 1733–1737. [View Article] [PubMed]
LiuY.,
JinJ.-H.,
LiuY.-H.,
ZhouY.-G.,
LiuZ.-P.(2010).Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent. . Int J Syst Evol Microbiol60, 2780–2785. [View Article] [PubMed]
LiuM.,
DaiJ.,
LiuY.,
CaiF.,
WangY.,
RahmanE.,
FangC.(2011).Desertibacter roseus gen. nov., sp. nov., a gamma radiation-resistant bacterium in the family Rhodospirillaceae, isolated from desert sand. . Int J Syst Evol Microbiol61, 1109–1113. [View Article] [PubMed]
López-LópezA.,
PujalteM. J.,
BenllochS.,
Mata-RoigM.,
Rosselló-MoraR.,
GarayE.,
Rodríguez-ValeraF.(2002).Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria
. . Int J Syst Evol Microbiol52, 1277–1283. [View Article] [PubMed]
MandelM.,
MarmurJ.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol12B, 195–206. [View Article]
MaszenanA. M.,
SeviourR. J.,
PatelB. K. C.,
JanssenP. H.,
WannerJ.(2005).Defluvicoccus vanus gen. nov., sp. nov., a novel Gram-negative coccus/coccobacillus in the ‘Alphaproteobacteria’ from activated sludge. . Int J Syst Evol Microbiol55, 2105–2111. [View Article] [PubMed]
PfennigN.,
LünsdorfH.,
SülingJ.,
ImhoffJ. F.(1997).Rhodospira trueperi gen. nov., spec. nov., a new phototrophic Proteobacterium of the alpha group. . Arch Microbiol168, 39–45. [View Article] [PubMed]
SchleiferK. H.,
SchulerD.,
SpringS.,
WeizeneggerM.,
AmannR.,
LudwigW.,
KohlerM.(1991). The genus Magnetospirillum gen. nov., description of Magnetospirillum gryphiswaldense sp, nov. and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum comb. nov.. Syst Appl Microbiol14, 379–385. [View Article]
SlyL. I.,
StackebrandtE.(1999). Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum
. . Int J Syst Bacteriol49, 541–544. [View Article]
TarrandJ. J.,
KriegN. R.,
DöbereinerJ.(1978). A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.. Can J Microbiol24, 967–980. [View Article] [PubMed]
UriosL.,
MichoteyV.,
IntertagliaL.,
LesongeurF.,
LebaronP.(2008).Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea. . Int J Syst Evol Microbiol58, 2336–2341. [View Article] [PubMed]
YoonJ. H.,
KimH.,
KimS. B.,
KimH. J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY. H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol46, 502–505. [View Article]
ZhangG. I.,
HwangC. Y.,
ChoB. C.(2008).Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. . Int J Syst Evol Microbiol58, 479–485. [View Article] [PubMed]