1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A could grow at 20–42 °C, pH 6.0–10.0 and tolerate up to 7 % (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A showed pairwise sequence similarity to LMG 1223 (97.7 %), ATCC 14909 (97.8 %), DSM 17744 (97.8 %), JCM 15604 (97.6 %), DSM 21016 (97.6 %) and BCRC 17807 (97.5 %), and lower sequence similarity to other species of the genus . According to DNA–DNA association analysis, the relatedness of strain CC-G9A to BCRC 10458, DSM 17744, BCRC 11893, DSM 21016, BCRC 17807 and BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8 %, respectively. The evolutionary trees reconstructed based on 16S rRNA, and gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C 3-OH, C C 3-OH, C and summed features 3 and 8 consisting of Cω7/Cω6 and Cω7/Cω6, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A is proposed to represent a novel species within the genus for which the name sp. nov. is proposed. The type strain is CC-G9A ( = BCRC 80438 = JCM 18416).

Funding
This study was supported by the:
  • , National Science Council
  • , Council of Agriculture
  • , Executive Yuan
  • , Ministry of Education, Taiwan, ROC
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2013-12-01
2020-07-10
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