1887

Abstract

A Gram-stain-negative, rod-shaped, strictly aerobic, flagellated and non-spore-forming marine bacterium designated strain CC-AMO-30B was isolated from coastal surface seawater, Taiwan. Strain CC-AMO-30B synthesized astaxanthin [40 µg (g dry weight)] and formed reddish-orange-coloured colonies on marine agar (Difco 2216). The strain showed highest pairwise 16S rRNA gene sequence similarity to CC-TBT-3 (96.4 %) followed by other members of the family (<94 %) and established a discrete phyletic lineage associated with the former. The polar lipid profile constituted a remarkable number of unidentified glycolipids (GL1–8), in addition to diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and two unidentified lipids (L1–2). The major fatty acids (>5 % of total fatty acids) were Cω7/Cω6 (summed feature 8), Cω7/Cω6 (summed feature 3), C 2-OH, methyl Cω7, Cω6 and C. DNA G+C content was 70.6 %; major respiratory quinone was ubiquinone Q-10; predominant polyamine was the triamine -homospermidine. Chemotaxonomic evidence including characteristic glycolipid profile, presence of significant amounts of C 2-OH and absence of typical hydroxylated fatty acids such as C 2-OH, C 2-OH and C 2-OH in considerable amounts, accompanied by phylogenetic distinctiveness and several other phenotypic features support the classification of strain CC-AMO-30B as a representative of a novel species within the genus for which the name sp. nov. is proposed; the type strain is CC-AMO-30B ( = JCM 18551 = BCRC 80465).

Funding
This study was supported by the:
  • Ministry of Education, Taiwan, R.O.C.
  • Ministry of Economic Affairs, Taiwan
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2013-09-01
2021-07-28
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