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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

sp. nov., a sulfate-reducing bacterium isolated from the subsurface environment of a landfill Free

Abstract

A novel sulfate-reducing, strictly anaerobic and endospore-forming bacterium, designated strain A5LFS102, was isolated from a subsurface landfill sample. The strain was characterized using a polyphasic approach. Optimal growth was observed at 37 °C and pH 7.5 with sulfate as an electron acceptor. Sulfite and thiosulfate were utilized as electron acceptors. The respiratory isoprenoid quinone was menaquinone MK-7. 16S rRNA gene sequence analysis assigned strain A5LFS102 to the genus . Both 16S rRNA and dissimilatory sulfate reductase () genes were compared with those of representative members of the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A5LFS102 was closely related to DSM 10349 (94.6 % sequence similarity). The G+C content of the DNA was 45.4 mol%. The total cellular fatty acid profile was dominated by C fatty acids. These phenotypic and genotypic data showed that strain A5LFS102 should be recognized as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A5LFS102 ( = DSM 23699 = JCM 14036 = MTCC 7767).

  • Published Online:
Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR)
  • Department of Biotechnology (DBT), Government of India
© 2013 IUMS
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2013-06-01
2024-04-24
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Desulfotomaculum defluvii sp. nov., a sulfate-reducing bacterium isolated from the subsurface environment of a landfill
Int J Syst Evol Microbiol 63, 2290 (2013); https://doi.org/10.1099/ijs.0.047662-0
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