1887

Abstract

A novel sulfate-reducing, strictly anaerobic and endospore-forming bacterium, designated strain A5LFS102, was isolated from a subsurface landfill sample. The strain was characterized using a polyphasic approach. Optimal growth was observed at 37 °C and pH 7.5 with sulfate as an electron acceptor. Sulfite and thiosulfate were utilized as electron acceptors. The respiratory isoprenoid quinone was menaquinone MK-7. 16S rRNA gene sequence analysis assigned strain A5LFS102 to the genus . Both 16S rRNA and dissimilatory sulfate reductase () genes were compared with those of representative members of the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A5LFS102 was closely related to DSM 10349 (94.6 % sequence similarity). The G+C content of the DNA was 45.4 mol%. The total cellular fatty acid profile was dominated by C fatty acids. These phenotypic and genotypic data showed that strain A5LFS102 should be recognized as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A5LFS102 ( = DSM 23699 = JCM 14036 = MTCC 7767).

Funding
This study was supported by the:
  • , Council of Scientific and Industrial Research (CSIR)
  • , Department of Biotechnology (DBT), Government of India
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2013-06-01
2020-08-04
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References

  1. Alazard D., Joseph M., Battaglia-Brunet F., Cayol J.-L., Ollivier B. ( 2010 ). Desulfosporosinus acidiphilus sp. nov.: a moderately acidophilic sulfate-reducing bacterium isolated from acid mining drainage sediments. . Extremophiles 14, 305312. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  3. Burkhardt E. M., Bischoff S., Akob D. M., Büchel G., Küsel K. ( 2011 ). Heavy metal tolerance of Fe(III)-reducing microbial communities in contaminated creek bank soils. . Appl Environ Microbiol 77, 31323136. [CrossRef] [PubMed]
    [Google Scholar]
  4. Campbell L. L., Singleton R. ( 1986 ). Genus Desulfotomaculum Campbell and Postgate 1965, 361AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 12001202. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  5. Gibbons N. E., Murray R. G. E. ( 1978 ). Proposals concerning the higher taxa of bacteria. . Int J Syst Bacteriol 28, 16. [CrossRef]
    [Google Scholar]
  6. Hagenauer A., Hippe H., Rainey F. A. ( 1997 ). Desulfotomaculum aeronauticum sp. nov., a sporeforming, thiosulfate-reducing bacterium from corroded aluminium alloy in an aircraft. . Syst Appl Microbiol 20, 6571. [CrossRef]
    [Google Scholar]
  7. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  8. Haouari O., Fardeau M. L., Cayol J. L., Casiot C., Elbaz-Poulichet F., Hamdi M., Joseph M., Ollivier B. ( 2008 ). Desulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial Tunisian hot spring. . Int J Syst Evol Microbiol 58, 25292535. [CrossRef] [PubMed]
    [Google Scholar]
  9. Henry E. A., Devereux R., Maki J. S., Gilmour C. C., Woese C. R., Mandelco L., Schauder R., Remsen C. C., Mitchell R. ( 1994 ). Characterization of a new thermophilic sulfate-reducing bacterium Thermodesulfovibrio yellowstonii, gen. nov. and sp. nov.: its phylogenetic relationship to Thermodesulfobacterium commune and their origins deep within the bacterial domain. . Arch Microbiol 161, 6269. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jørgensen B. B. ( 1977 ). The sulfur cycle of a coastal marine sediment (Limfjorden, Denmark). . Limnol Oceanogr 22, 814832. [CrossRef]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Klein M., Friedrich M., Roger A. J., Hugenholtz P., Fishbain S., Abicht H., Blackall L. L., Stahl D. A., Wagner M. ( 2001 ). Multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes. . J Bacteriol 183, 60286035. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kuever J., Rainey F. A., Hippe H. ( 1999 ). Description of Desulfotomaculum sp. Groll as Desulfotomaculum gibsoniae sp. nov.. Int J Syst Bacteriol 49, 18011808. [CrossRef] [PubMed]
    [Google Scholar]
  15. Liu Y., Karnauchow T. M., Jarrell K. F., Balkwill D. L., Drake G. R., Ringelberg D., Clarno R., Boone D. R. ( 1997 ). Description of two new thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a deep terrestrial subsurface and Desulfotomaculum luciae sp. nov., from a hot spring. . Int J Syst Bacteriol 47, 615621. [CrossRef]
    [Google Scholar]
  16. Lovley D. R., Roden E. E., Phillips E. J. P., Woodward J. C. ( 1993 ). Enzymatic iron and uranium reduction by sulfate reducing bacteria. . Mar Geol 113, 4153. [CrossRef]
    [Google Scholar]
  17. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12, 195206. [CrossRef]
    [Google Scholar]
  18. Manz W., Eisenbrecher M., Neu T. R., Szewzy K. ( 1998 ). Abundance and spatial organization of Gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides. . FEMS Microbiol Ecol 25, 4361. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  20. Moreau J. W., Zierenberg R. A., Banfield J. F. ( 2010 ). Diversity of dissimilatory sulfite reductase genes (dsrAB) in a salt marsh impacted by long-term acid mine drainage. . Appl Environ Microbiol 76, 48194828. [CrossRef] [PubMed]
    [Google Scholar]
  21. Parshina S. N., Sipma J., Nakashimada Y., Henstra A. M., Smidt H., Lysenko A. M., Lens P. N. L., Lettinga G., Stams A. J. M. ( 2005 ). Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100% CO. . Int J Syst Evol Microbiol 55, 21592165. [CrossRef] [PubMed]
    [Google Scholar]
  22. Pikuta E., Lysenko A., Suzina N., Osipov G., Kuznetsov B., Tourova T., Akimenko V., Laurinavichius K. ( 2000 ). Desulfotomaculum alkaliphilum sp. nov., a new alkaliphilic, moderately thermophilic, sulfate-reducing bacterium. . Int J Syst Evol Microbiol 50, 2533. [CrossRef] [PubMed]
    [Google Scholar]
  23. Raskin L., Rittmann B. E., Stahl D. A. ( 1996 ). Competition and coexistence of sulfate-reducing and methanogenic populations in anaerobic biofilms. . Appl Environ Microbiol 62, 38473857.[PubMed]
    [Google Scholar]
  24. Rogosa M. ( 1971 ). Peptococcaceae, a new family to include the gram positive, anaerobic cocci of the genera Peptococcus, Peptostreptococcus and Ruminococcus . . Int J Syst Bacteriol 21, 234237. [CrossRef]
    [Google Scholar]
  25. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  26. Stackebrandt E., Sproer C., Rainey F. A., Burghardt J., Päuker O., Hippe H. ( 1997 ). Phylogenetic analysis of the genus Desulfotomaculum: evidence for the misclassification of Desulfotomaculum guttoideum and description of Desulfotomaculum orientis as Desulfosporosinus orientis gen. nov., comb. nov.. Int J Syst Bacteriol 47, 11341139. [CrossRef] [PubMed]
    [Google Scholar]
  27. Suresh K., Mayilraj S., Chakrabarti T. ( 2006 ). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’. . Int J Syst Evol Microbiol 56, 17031707. [CrossRef] [PubMed]
    [Google Scholar]
  28. Suresh K., Prakash D., Rastogi N., Jain R. K. ( 2007 ). Clostridium nitrophenolicum sp. nov., a novel anaerobic p-nitrophenol-degrading bacterium, isolated from a subsurface soil sample. . Int J Syst Evol Microbiol 57, 18861890. [CrossRef] [PubMed]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tebo B. M., Obraztsova A. Y. ( 1998 ). Sulfate-reducing bacterium grows with Cr(VI), U(VI), Mn(IV) and Fe(III) as electron acceptors. . FEMS Microbiol Lett 162, 193198. [CrossRef]
    [Google Scholar]
  31. Vandieken V., Knoblauch C., Jørgensen B. B. ( 2006 ). Desulfotomaculum arcticum sp. nov., a novel spore-forming, moderately thermophilic, sulfate-reducing bacterium isolated from a permanently cold fjord sediment of Svalbard. . Int J Syst Evol Microbiol 56, 687690. [CrossRef] [PubMed]
    [Google Scholar]
  32. Wagner M., Roger A. J., Flax J. L., Brusseau G. A., Stahl D. A. ( 1998 ). Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. . J Bacteriol 180, 29752982.[PubMed]
    [Google Scholar]
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