1887

Abstract

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the clade and to be most closely related to , a species recently discovered in a mangrove forest in Thailand. The species name of sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004 ( = CBS 12653 = NCYC 3782) designated as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047555-0
2013-01-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/1/393.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047555-0&mimeType=html&fmt=ahah

References

  1. Barbosa A. C., Cadete R. M., Gomes F. C. O., Lachance M.-A., Rosa C. A.. ( 2009;). Candida materiae sp. nov., a yeast species isolated from rotting wood in the Atlantic Rain Forest. . Int J Syst Evol Microbiol 59:, 2104–2106. [CrossRef][PubMed]
    [Google Scholar]
  2. Cadete R. M., Santos R. O., Melo M. A., Mouro A., Gonçalves D. L., Stambuk B. U., Gomes F. C. O., Lachance M.-A., Rosa C. A.. ( 2009;). Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. . FEMS Yeast Res 9:, 1338–1342. [CrossRef][PubMed]
    [Google Scholar]
  3. Chang C.-F., Lin Y.-C., Chen S.-F., Carvajal Barriga E. J., Barahona P. P., James S. A., Bond C. J., Roberts I. N., Lee C.-F.. ( 2012;). Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade. . Int J Food Microbiol 153:, 10–14. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. James S. A., Collins M. D., Roberts I. N.. ( 1996;). Use of an rRNA internal transcribed spacer region to distinguish phylogenetically closely related species of the genera Zygosaccharomyces and Torulaspora. . Int J Syst Bacteriol 46:, 189–194. [CrossRef][PubMed]
    [Google Scholar]
  6. James S. A., Carvajal Barriga E. J., Bond C. J., Cross K., Núñez N. C., Portero P. B., Roberts I. N.. ( 2009;). Candida carvajalis sp. nov., an ascomycetous yeast species from the Ecuadorian Amazon jungle. . FEMS Yeast Res 9:, 784–788. [CrossRef][PubMed]
    [Google Scholar]
  7. James S. A., Cadet G. M., Carvajal Barriga E. J., Barahona P. P., Cross K., Bond C. J., Roberts I. N.. ( 2011;). Saturnispora quitensis sp. nov., a yeast species isolated from the Maquipucuna cloud forest reserve in Ecuador. . Int J Syst Evol Microbiol 61:, 3072–3076. [CrossRef][PubMed]
    [Google Scholar]
  8. Jindamorakot S., Limtong S., Yongmanitchai W., Tuntirungkij M., Potacharoen W., Kawasaki H., Nakase T.. ( 2007;). Two new anamorphic yeasts, Candida thailandica sp. nov. and Candida lignicola sp. nov., isolated from insect frass in Thailand. . FEMS Yeast Res 7:, 1409–1414. [CrossRef][PubMed]
    [Google Scholar]
  9. Kurtzman C. P., Fell J. W., Boekhout T., Robert V.. ( 2011;). Methods for isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 87–110. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  10. Lachance M.-A.. ( 2011;). Metschnikowia Kamienski. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 575–620. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  11. Lachance M.-A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P.. ( 2011;). Candida Berkhout. . In The Yeasts, a Taxonomic Study, , 5th edn., pp. 987–1278. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  12. Libkind D., Hittinger C. T., Valério E., Gonçalves C., Dover J., Johnston M., Gonçalves P., Sampaio J. P.. ( 2011;). Microbe domestication and the identification of the wild genetic stock of lager-brewing yeast. . Proc Natl Acad Sci U S A 108:, 14539–14544. [CrossRef][PubMed]
    [Google Scholar]
  13. Limtong S., Yongmanitchai W.. ( 2010;). Candida chanthaburiensis sp. nov., Candida kungkrabaensis sp. nov. and Candida suratensis sp. nov., three novel yeast species from decaying plant materials submerged in water of mangrove forests. . Antonie van Leeuwenhoek 98:, 379–388. [CrossRef][PubMed]
    [Google Scholar]
  14. Morais P. B., Lachance M.-A., Rosa C. A.. ( 2005;). Saturnispora hagleri sp. nov., a yeast species isolated from Drosophila flies in Atlantic rainforest in Brazil. . Int J Syst Evol Microbiol 55:, 1725–1727. [CrossRef][PubMed]
    [Google Scholar]
  15. Nguyen N. H., Suh S.-O., Blackwell M.. ( 2007;). Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. . Mycologia 99:, 842–858. [CrossRef][PubMed]
    [Google Scholar]
  16. O’Donnell K.. ( 1993;). Fusarium and its near relatives. . In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 225–233. Edited by Reynolds D. R., Taylor J. W... Wallingford, UK:: CAB International;.
    [Google Scholar]
  17. Pearson W. R., Lipman D. J.. ( 1988;). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85:, 2444–2448. [CrossRef][PubMed]
    [Google Scholar]
  18. Ramírez C., González A.. ( 1984a;). Rhodotorula grinbergsii sp. nov. isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. . Mycopathologia 88:, 51–53. [CrossRef]
    [Google Scholar]
  19. Ramírez C., González A.. ( 1984b;). Five new filamentous, glucose-fermenting Candida isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. . Mycopathologia 88:, 83–92. [CrossRef]
    [Google Scholar]
  20. Rosa C. A., Pagnocca F. C., Lachance M.-A., Ruivo C. C. C., Medeiros A. O., Pimentel M. R. C., Fontenelle J. C. R., Martins R. P.. ( 2007;). Candida flosculorum sp. nov. and Candida floris sp. nov., two yeast species associated with tropical flowers. . Int J Syst Evol Microbiol 57:, 2970–2974. [CrossRef][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sipiczki M.. ( 2011;). Dimorphic cycle in Candida citri sp. nov., a novel yeast species isolated from rotting fruit in Borneo. . FEMS Yeast Res 11:, 202–208. [CrossRef][PubMed]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  24. White T. J., Bruns T., Lee S., Taylor J. W.. ( 1990;). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Applications, pp. 315–322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J... New York:: Academic Press;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047555-0
Loading
/content/journal/ijsem/10.1099/ijs.0.047555-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error