1887

Abstract

A novel, Gram-stain-positive, non-motile, facultatively anaerobic, rod- or coccoid-shaped bacterium, designated strain ORY33, was isolated from the gut of a camel cricket (). The 16S rRNA gene sequence analysis showed that strain ORY33 belonged to the genus , with highest sequence similarity to S-5623 (97.7 %). The strain grew optimally at 30 °C and pH 7 in the presence of 0 % (w/v) NaCl. Catalase and oxidase activities were negative. The genomic DNA G+C content of strain ORY33 was 45.1 mol%. DNA–DNA hybridization values between strain ORY33 and closely related members of the genus were less than 27 %. The major fatty acids of strain ORY33 were Cω9, C and C. Based on these phenotypic, phylogenetic and genotypic analyses, strain ORY33 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is ORY33 ( = KACC 16890 = JCM 18559).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • National Research Foundation of Korea (Award 2011-0028854)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.047548-0
2013-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/8/2951.html?itemId=/content/journal/ijsem/10.1099/ijs.0.047548-0&mimeType=html&fmt=ahah

References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. ( 2005 ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71, 88258835. [View Article] [PubMed]
    [Google Scholar]
  2. Bousfield G. R., Sugino H., Ward D. N. ( 1985 ). Demonstration of a COOH-terminal extension on equine lutropin by means of a common acid-labile bond in equine lutropin and equine chorionic gonadotropin. . J Biol Chem 260, 95319533.[PubMed]
    [Google Scholar]
  3. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. ( 2008 ). Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75, 523530. [View Article] [PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  5. Collins M. D., Samelis J., Metaxopoulos J., Wallbanks S. ( 1993 ). Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. . J Appl Bacteriol 75, 595603. [View Article] [PubMed]
    [Google Scholar]
  6. De Bruyne K., Schillinger U., Caroline L., Boehringer B., Cleenwerck I., Vancanneyt M., De Vuyst L., Franz C. M., Vandamme P. ( 2007 ). Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species. . Int J Syst Evol Microbiol 57, 29522959. [View Article] [PubMed]
    [Google Scholar]
  7. De Bruyne K., Camu N., Lefebvre K., De Vuyst L., Vandamme P. ( 2008 ). Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation. . Int J Syst Evol Microbiol 58, 27212725. [View Article] [PubMed]
    [Google Scholar]
  8. Dillon R. J., Dillon V. M. ( 2004 ). The gut bacteria of insects: nonpathogenic interactions. . Annu Rev Entomol 49, 7192. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  10. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  11. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598. [View Article] [PubMed]
    [Google Scholar]
  12. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [View Article]
    [Google Scholar]
  13. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  14. Lee J. S., Lee K. C., Ahn J. S., Mheen T. I., Pyun Y. R., Park Y. H. ( 2002 ). Weissella koreensis sp. nov., isolated from kimchi. . Int J Syst Evol Microbiol 52, 12571261. [View Article] [PubMed]
    [Google Scholar]
  15. MIDI (1999). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  16. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005 ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  17. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. ( 1992 ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79, 5965.[PubMed] [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. ( 1989 ). Molecular cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor:: NY Cold Spring Harbor Laboratory;.
    [Google Scholar]
  20. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  21. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  23. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575580.[PubMed]
    [Google Scholar]
  24. Vilmos P., Kurucz E. ( 1998 ). Insect immunity: evolutionary roots of the mammalian innate immune system. . Immunol Lett 62, 5966. [View Article] [PubMed]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.047548-0
Loading
/content/journal/ijsem/10.1099/ijs.0.047548-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error