1887

Abstract

An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3EN, was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3–2.4 µm long by 0.30–0.75 µm wide) with single flagella. The strain grew at 10–37 °C (optimum 25–30 °C) and at pH 4.5–9.5 (optimum 5.0–7.0). The major cellular fatty acids were C, Cω7 11-methyl, C 3-OH and summed feature 8 (comprising Cω7/Cω6). The genomic DNA G+C content of strain CGM1-3EN was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3EN was identified as DSM 4723 (97.2 % 16S rRNA gene sequence similarity) and the DNA–DNA hybridization value between strain CGM1-3EN and DSM 4723 was less than 24.5 %. Strain CGM1-3EN used -glucose, -fructose, sucrose, maltose, trehalose, -mannose, -mannitol, -sorbitol, -galactose, cellobiose, lactose, raffinose, fumarate, pyruvate, -alanine and glycerol as carbon sources. Based on data from the present polyphasic study, the forest soil isolate CGM1-3EN is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CGM1-3EN ( = AIM0088 = KCTC 32102 = JCM 18544).

Funding
This study was supported by the:
  • , National Research Foundation of Korea , (Award NRF-2009-C1AAA001-0093286)
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2013-10-01
2021-02-26
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