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Volume 63, Issue Pt_7

sp. nov., isolated from environmental water Free

Abstract

Strain Eri-1 was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus , but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6±1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1 grew in Korthof’s medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1 within the radiation of the genus where it formed a unique lineage within the clade of the known saprophytic species of the genus . The strain was not pathogenic to hamsters. Strain Eri-1 exhibited low levels (11.2–12.6 %) of similarity by DNA–DNA hybridization to the three most closely related species of the genus . The DNA G+C content of the genome of strain Eri-1 was 42.5±0.1 mol%. These results suggest that strain Eri-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Eri-1 ( = DSM 26084 = JCM 18486).

  • Published Online:
Funding
This study was supported by the:
  • Special Coordination Funds on Science and Technology of the Ministry of Education, Sports, Culture, Science and Technology (MEXT) of Japan
  • Science and Technology Research Partnership for Sustainable Development (SATREPS)
  • Japan Science and Technology Agency (JST)
  • Japan International Cooperation Agency (JICA)
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2013-07-01
2024-04-23
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References

  1. Adler B., de la Peña Moctezuma A. ( 2010 ). Leptospira and leptospirosis. . Vet Microbiol 140, 287–296. [View Article] [PubMed]
    [Google Scholar]
  2. Brendle J. J., Alexander A. D. ( 1974 ). Contamination of bacteriological media by Leptospira biflexa . . Appl Microbiol 28, 505–506. [PubMed]
    [Google Scholar]
  3. Brenner D. J., Kaufmann A. F., Sulzer K. R., Steigerwalt A. G., Rogers F. C., Weyant R. S. ( 1999 ). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. . Int J Syst Bacteriol 49, 839–858. [View Article] [PubMed]
    [Google Scholar]
  4. Carleton O., Charon N. W., Allender P., O’Brien S. ( 1979 ). Helix handedness of Leptospira interrogans as determined by scanning electron microscopy. . J Bacteriol 137, 1413–1416. [PubMed]
    [Google Scholar]
  5. Chakraborty A., Miyahara S., Villanueva S. Y., Saito M., Gloriani N. G., Yoshida S. ( 2011 ). A novel combination of selective agents for isolation of Leptospira species. . Microbiol Immunol 55, 494–501. [View Article] [PubMed]
    [Google Scholar]
  6. Dikken H., Kmety E. ( 1978 ). Serological typing methods of leptospires. . Methods Microbiol 11, 259–307. [View Article]
    [Google Scholar]
  7. Graves S., Faine S. ( 1970 ). Antileptospiral agglutinins produced in rabbits. . Bull World Health Organ 43, 579–587. [PubMed]
    [Google Scholar]
  8. Inada R., Ido Y., Hoki R., Kaneko R., Ito H. ( 1916 ). The etiology, mode of infection, and specific therapy of Weil's disease (spirochaetosis icterohaemorrhagica). . J Exp Med 23, 377–402. [View Article] [PubMed]
    [Google Scholar]
  9. Johnson R. C., Rogers P. ( 1964 ). Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. . J Bacteriol 88, 1618–1623. [PubMed]
    [Google Scholar]
  10. Kawabata H., Dancel L. A., Villanueva S. Y., Yanagihara Y., Koizumi N., Watanabe H. ( 2001 ). flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp. . Microbiol Immunol 45, 491–496. [PubMed] [CrossRef]
    [Google Scholar]
  11. Kawamura Y., Whiley R. A., Shu S. E., Ezaki T., Hardie J. M. ( 1999 ). Genetic approaches to the identification of the mitis group within the genus Streptococcus . . Microbiology 145, 2605–2613. [PubMed]
    [Google Scholar]
  12. Levett P. N. ( 2001 ). Leptospirosis. . Clin Microbiol Rev 14, 296–326. [View Article] [PubMed]
    [Google Scholar]
  13. Levett P. N., Morey R. E., Galloway R., Steigerwalt A. G., Ellis W. A. ( 2005 ). Reclassification of Leptospira parva Hovind-Hougen et al. 1982 as Turneriella parva gen. nov., comb. nov.. Int J Syst Evol Microbiol 55, 1497–1499. [View Article] [PubMed]
    [Google Scholar]
  14. Levett P. N., Morey R. E., Galloway R. L., Steigerwalt A. G. ( 2006 ). Leptospira broomii sp. nov., isolated from humans with leptospirosis. . Int J Syst Evol Microbiol 56, 671–673. [View Article] [PubMed]
    [Google Scholar]
  15. Matthias M. A., Ricaldi J. N., Cespedes M., Diaz M. M., Galloway R. L., Saito M., Steigerwalt A. G., Patra K. P., Ore C. V. & other authors ( 2008 ). Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. . PLoS Negl Trop Dis 2, e213. [View Article] [PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  17. Murgia R., Riquelme N., Baranton G., Cinco M. ( 1997 ). Oligonucleotides specific for pathogenic and saprophytic leptospira occurring in water. . FEMS Microbiol Lett 148, 27–34. [View Article] [PubMed]
    [Google Scholar]
  18. Page R. D. ( 1996 ). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12, 357–358. [PubMed]
    [Google Scholar]
  19. Ramadass P., Jarvis B. D. W., Corner R. J., Penny D., Marshall R. B. ( 1992 ). Genetic characterization of pathogenic Leptospira species by DNA hybridization. . Int J Syst Bacteriol 42, 215–219. [View Article] [PubMed]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425. [PubMed]
    [Google Scholar]
  21. Slack A. T., Kalambaheti T., Symonds M. L., Dohnt M. F., Galloway R. L., Steigerwalt A. G., Chaicumpa W., Bunyaraksyotin G., Craig S. & other authors ( 2008 ). Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand. . Int J Syst Evol Microbiol 58, 2305–2308. [View Article] [PubMed]
    [Google Scholar]
  22. Slack A. T., Khairani-Bejo S., Symonds M. L., Dohnt M. F., Galloway R. L., Steigerwalt A. G., Bahaman A. R., Craig S., Harrower B. J., Smythe L. D. ( 2009 ). Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia. . Int J Syst Evol Microbiol 59, 705–708. [View Article] [PubMed]
    [Google Scholar]
  23. Smythe L., Adler B., Hartskeerl R. A., Galloway R. L., Turenne C. Y., Levett P. N. International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Leptospiraceae ( 2012 ). Classification of Leptospira genomospecies 1, genomospecies 3, genomospecies 4 and genomospecies 5 as Leptospira alstonii sp. nov., Leptospira vanthielii sp. nov., Leptospira terpstrae sp. nov. and Leptospira yanagawae sp. nov., respectively. . Int J Syst Evol Microbiol 63, 1859–1862. [View Article] [PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  25. Tomida J., Sauer S., Morita Y., Ezaki T., Kawamura Y. ( 2011 ). Streptococcus fryi sp. nov., a novel species with Lancefield group M antigens. . FEMS Microbiol Lett 314, 95–100. [View Article] [PubMed]
    [Google Scholar]
  26. Villanueva S. Y., Ezoe H., Baterna R. A., Yanagihara Y., Muto M., Koizumi N., Fukui T., Okamoto Y., Masuzawa T. & other authors ( 2010 ). Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines. . Am J Trop Med Hyg 82, 889–898. [View Article] [PubMed]
    [Google Scholar]
  27. WHO & ILS ( 2003 ). Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Geneva, Switzerland:: World Health Organization;.
     [Google Scholar]
  28. Wilson K. ( 1987 ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Green Publishing & Wiley-Interscience;.
     [Google Scholar]
  29. Yasuda P. H., Steigerwalt A. G., Sulzer C. R., Kaufmann A. F., Rogers F. C., Brenner D. J. ( 1987 ). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. . Int J Syst Bacteriol 37, 407–415. [View Article]
    [Google Scholar]
  30. Zuerner R. L. ( 2011 ). Genus I. Leptospira Family IV. Leptospiraceae Phylum XV. Spirochaetes phyl. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 546–556. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L, Ludwig W., Whitman W. B. . New York:: Springer;.
     [Google Scholar]
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Leptospiraidonii sp. nov., isolated from environmental water
Int J Syst Evol Microbiol 63, 2457 (2013); https://doi.org/10.1099/ijs.0.047233-0
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