1887

Abstract

Strain Eri-1 was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus , but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6±1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1 grew in Korthof’s medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1 within the radiation of the genus where it formed a unique lineage within the clade of the known saprophytic species of the genus . The strain was not pathogenic to hamsters. Strain Eri-1 exhibited low levels (11.2–12.6 %) of similarity by DNA–DNA hybridization to the three most closely related species of the genus . The DNA G+C content of the genome of strain Eri-1 was 42.5±0.1 mol%. These results suggest that strain Eri-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Eri-1 ( = DSM 26084 = JCM 18486).

Funding
This study was supported by the:
  • , Special Coordination Funds on Science and Technology of the Ministry of Education, Sports, Culture, Science and Technology (MEXT) of Japan
  • , Science and Technology Research Partnership for Sustainable Development (SATREPS)
  • , Japan Science and Technology Agency (JST)
  • , Japan International Cooperation Agency (JICA)
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2013-07-01
2020-10-24
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