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Volume 63, Issue Pt_8

sp. nov., an extremely halophilic actinomycete isolated from a salt lake Free

Abstract

A novel halophilic, filamentous actinobacterium, designated strain TRM 40139, was isolated from a hypersaline habitat in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation of the genus and the organism was related most closely to the type strains of (97.6 % similarity), (97.6 %) and (97.1 %). However, it had relatively lower mean DNA–DNA relatedness values with the above strains (36.4, 31.3 and 26.1 %, respectively). Optimal growth occurred at 35 °C, at pH 7.0 and in the presence of 12 % (w/v) NaCl. The whole-cell sugar pattern consisted of xylose, glucose, ribose and arabinose. The major fatty acids were iso-C (28.0 %) and anteiso-C (27.6 %). The diagnostic phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown phospholipids. The predominant menaquinones were MK-9(H) (49.8 %) and MK-10(H) (24.2 %). The G+C content of the genomic DNA was 66.4 mol%. Strain TRM 40139 therefore represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 40139 ( = KCTC 19657 = CCTCC AA 2012020).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31060001 and 31060003)
  • Key Research Fund Project of Xihua University (Award Z1220530)
  • Key Project for Department of Science and Technology of Ministry of Education of China (Award 209145)
  • 973 Program (Award 2010CB134500)
© 2013 IUMS
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2013-08-01
2024-04-24
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References

  1. Berd D. ( 1973 ). Laboratory identification of clinically important aerobic actinomycetes. . Appl Microbiol 25, 665–681.[PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–789. [View Article]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  7. Gochnauer M. B., Leppard G. G., Komaratat P., Kates M., Novitsky T., Kushner D. J. ( 1975 ). Isolation and characterization of Actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete. . Can J Microbiol 21, 1500–1511. [View Article] [PubMed]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 54–63. [View Article]
    [Google Scholar]
  9. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  10. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  11. Guan T.-W., Liu Y., Zhao K., Xia Z.-F., Zhang X.-P., Zhang L.-L. ( 2010a ). Actinopolyspora xinjiangensis sp. nov., a novel extremely halophilic actinomycete isolated from a salt lake in Xinjiang, China. . Antonie van Leeuwenhoek 98, 447–453. [View Article] [PubMed]
    [Google Scholar]
  12. Guan T.-W., Xiao J., Zhao K., Luo X.-X., Zhang X.-P., Zhang L.-L. ( 2010b ). Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 60, 349–352. [View Article] [PubMed]
    [Google Scholar]
  13. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  14. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  15. Kelly K. L. ( 1964 ). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  16. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.-S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  17. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  18. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 1424–1428. [View Article] [PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  20. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221–233. [View Article]
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  22. Ruan J.-S., Al-tai A. M., Zhou Z.-H., Qu L.-H. ( 1994 ). Actinopolyspora iraqiensis sp. nov., a new halophilic actinomycete isolated from soil. . Int J Syst Bacteriol 44, 759–763. [View Article]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  24. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  25. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  27. Tang S.-K., Wang Y., Klenk H. P., Shi R., Lou K., Zhang Y.-J., Chen C., Ruan J.-S., Li W.-J. ( 2011 ). Actinopolyspora alba sp. nov. and Actinopolyspora erythraea sp. nov., isolated from a salt field, and reclassification of Actinopolyspora iraqiensis Ruan et al. 1994 as a heterotypic synonym of Saccharomonospora halophila . . Int J Syst Evol Microbiol 61, 1693–1698. [View Article] [PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  29. Williams S. T. ( 1967 ). Sensitivity of streptomycetes to antibiotics as a taxonomic character. . J Gen Microbiol 46, 151–160. [View Article] [PubMed]
    [Google Scholar]
  30. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 1149–1153. [View Article] [PubMed]
    [Google Scholar]
  31. Yoshida M., Matsubara K., Kudo T., Horikoshi K. ( 1991 ). Actinopolyspora mortivallis sp. nov., a moderately halophilic actinomycete. . Int J Syst Bacteriol 41, 15–20. [View Article]
    [Google Scholar]
  32. Zhi X.-Y., Li W.-J., Stackebrandt E. ( 2009 ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59, 589–608. [View Article] [PubMed]
    [Google Scholar]
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Actinopolysporalacussalsi sp. nov., an extremely halophilic actinomycete isolated from a salt lake
Int J Syst Evol Microbiol 63, 3009 (2013); https://doi.org/10.1099/ijs.0.047167-0
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