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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_8

sp. nov., an extremely halophilic actinomycete isolated from a salt lake Free

Abstract

A novel halophilic, filamentous actinobacterium, designated strain TRM 40139, was isolated from a hypersaline habitat in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation of the genus and the organism was related most closely to the type strains of (97.6 % similarity), (97.6 %) and (97.1 %). However, it had relatively lower mean DNA–DNA relatedness values with the above strains (36.4, 31.3 and 26.1 %, respectively). Optimal growth occurred at 35 °C, at pH 7.0 and in the presence of 12 % (w/v) NaCl. The whole-cell sugar pattern consisted of xylose, glucose, ribose and arabinose. The major fatty acids were iso-C (28.0 %) and anteiso-C (27.6 %). The diagnostic phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown phospholipids. The predominant menaquinones were MK-9(H) (49.8 %) and MK-10(H) (24.2 %). The G+C content of the genomic DNA was 66.4 mol%. Strain TRM 40139 therefore represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 40139 ( = KCTC 19657 = CCTCC AA 2012020).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31060001 and 31060003)
  • Key Research Fund Project of Xihua University (Award Z1220530)
  • Key Project for Department of Science and Technology of Ministry of Education of China (Award 209145)
  • 973 Program (Award 2010CB134500)
© 2013 IUMS
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2013-08-01
2025-01-26
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Actinopolysporalacussalsi sp. nov., an extremely halophilic actinomycete isolated from a salt lake
Int J Syst Evol Microbiol 63, 3009 (2013); https://doi.org/10.1099/ijs.0.047167-0
/content/journal/ijsem/10.1099/ijs.0.047167-0
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