1887

Abstract

A Gram-stain-positive, aerobic, short rod-shaped bacterium, strain ZD1-4, was isolated from the Zhadang Glacier snow pit. The 16S rRNA gene sequence of the isolate showed highest similarity (98.8 %) to that of MB1-14. The major fatty acids of strain ZD1-4 were anteiso-C, C, C and anteiso-C. It possessed diphosphatidylglycerol as one of the major polar lipids, and MK-10 and MK-11 as the predominant isoprenoid quinones. The DNA G+C content of strain ZD1-4 was 63.8±0.2 mol% ( ). A number of phenotypic characteristics distinguished this bacterium from the type strains of other species of the genus . Moreover, the novel isolate showed only approximately 50 % DNA–DNA relatedness with MB1-14. According to these genotypic and phenotypic data, it is evident that strain ZD1-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZD1-4 ( = KACC 16570 = CGMCC 1.12042).

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2013-09-01
2019-12-11
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References

  1. Altenburger P., Kampfer P., Makristathis A., Lubitz W., Busse H. J.. ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Bacteriol 47:, 39–52. [CrossRef][PubMed]
    [Google Scholar]
  2. Bora N., Vancanneyt M., Gelsomino R., Snauwaert C., Swings J., Jones A. L., Ward A. C., Chamba J.-F., Kroppenstedt R. M.. & other authors ( 2008;). Mycetocola reblochoni sp. nov., isolated from the surface microbial flora of Reblochon cheese. . Int J Syst Evol Microbiol 58:, 2687–2693. [CrossRef][PubMed]
    [Google Scholar]
  3. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Corien B.. ( 2008;). Boundary Conditions for Microbial at Low Temperatures. . Psychrophiles: From Biodiversity to Biotechnology, pp. 17–28. Edited by Margesin R., Schinner F., Marx J. C., Gerday C... Berlin:: Springer;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  6. Embley T. M.. ( 1991;). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13:, 171–174. [CrossRef][PubMed]
    [Google Scholar]
  7. Hiraishi A., Ueda Y., Ishihara J.. ( 1998;). Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. . Appl Environ Microbiol 64:, 992–998.[PubMed]
    [Google Scholar]
  8. Huss V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  9. Jahnke K.-D.. ( 1992;). BASIC computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal 219 computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Luo X. S., Wang J., Zeng X. C., Wang Y. Q., Zhou L. L., Nie Y., Dai J., Fang C. X.. ( 2012;). Mycetocola manganoxydans sp. nov., an actinobacterium isolated from the Taklamakan desert. . Int J Syst Evol Microbiol 62:, 2967–2970. [CrossRef][PubMed]
    [Google Scholar]
  12. Mandel M., Igambi L., Bergendahl J., Dodson M. L. Jr, Scheltgen E.. ( 1970;). Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. . J Bacteriol 101:, 333–338.[PubMed]
    [Google Scholar]
  13. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  14. Reasoner D. J., Geldreich E. E.. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  15. Romano I., Lama L., Nicolaus B., Poli A., Gambacorta A., Giordano A.. ( 2006;). Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). . J Gen Appl Microbiol 52:, 339–348. [CrossRef][PubMed]
    [Google Scholar]
  16. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  17. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  19. Tindall B. J.. ( 1990;). Lipid-composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  20. Tsukamoto T., Takeuchi M., Shida O., Murata H., Shirata A.. ( 2001;). Proposal of Mycetocola gen. nov. in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp. nov., Mycetocola tolaasinivorans sp. nov. and Mycetocola lacteus sp. nov., isolated from cultivated mushroom, Pleurotus ostreatus. . Int J Syst Evol Microbiol 51:, 937–944. [CrossRef][PubMed]
    [Google Scholar]
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