1887

Abstract

Two new and phylogenetically closely related species in section are described and illustrated. Homothallic sp. nov. was isolated from New Jersey soil (USA) and is represented by the ex-type isolate NRRL 179 ( = CCF 4266 = Thom 4138.HS2 = IBT 31900). sp. nov. was isolated from water with high boracic acid anions content in Dukovany nuclear power station (Czech Republic). The sexual stage of this species is unknown, but the MAT1-1 locus was successfully amplified suggesting that the species is probably heterothallic and teleomorphic but is represented by only the ex-type isolate CCM 8003 ( = CCF 4037 = NRRL 62486 = IBT 31279 = IFM 60873). Both species can be distinguished from all previously described species in section based on morphology, maximum growth temperature, sequence data from five unlinked loci and unique secondary metabolites profiles.

Funding
This study was supported by the:
  • Ministry of Education, Youth and Sports of the Czech Republic
  • Charles University (Award GAUK 607812 and Research Project no. 265204/2012)
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2013-02-01
2024-11-13
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References

  1. Balajee S. A., Houbraken J., Verweij P. E., Hong S. B., Yaghuchi T., Varga J., Samson R. A. ( 2007 ). Aspergillus species identification in the clinical setting. . Stud Mycol 59, 3946. [View Article] [PubMed]
    [Google Scholar]
  2. Frisvad J. C., Thrane U. ( 1987 ). Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection). . J Chromatogr A 404, 195214. [View Article] [PubMed]
    [Google Scholar]
  3. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. ( 2010 ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59, 307321. [View Article] [PubMed]
    [Google Scholar]
  4. Hong S. B., Shin H. D., Hong J., Frisvad J. C., Nielsen P. V., Varga J., Samson R. A. ( 2008 ). New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati . . Antonie van Leeuwenhoek 93, 8798. [View Article] [PubMed]
    [Google Scholar]
  5. Houbraken J., Spierenburg H., Frisvad J. C. ( 2012 ). Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. . Antonie van Leeuwenhoek 101, 403421. [View Article] [PubMed]
    [Google Scholar]
  6. Hubka V. ( 2011 ). β- tubulin paralogs in Aspergillus: taxonomical importance and molecular tools for distinguishing. Diploma Thesis, Department of Botany, Charles University in Prague, Czech Republic (in Czech).
  7. Hubka V., Kolařík M. ( 2012 ). β-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing and taxonomic consequences. . Persoonia 29, 110. [CrossRef]
    [Google Scholar]
  8. Hubka V., Kubatova A., Mallatova N., Sedlacek P., Melichar J., Skorepova M., Mencl K., Lyskova P., Sramkova B. & other authors ( 2012 ). Rare and new aetiological agents revealed among 178 clinical Aspergillus strains obtained from Czech patients and characterised by molecular sequencing. . Med Mycol 50, 601610. [View Article] [PubMed]
    [Google Scholar]
  9. Jurjevic Z., Peterson S. W., Horn B. W. ( 2012 ). Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny. . IMA Fungus 3, 5979. [View Article]
    [Google Scholar]
  10. Nováková A., Hubka V., Saiz-Jimenez C., Kolarik M. ( 2012 ). Aspergillus baeticus sp. nov. and Aspergillus thesauricus sp. nov., two species in section Usti from Spanish caves. . Int J Syst Evol Microbiol 62, 27782785. [View Article] [PubMed]
    [Google Scholar]
  11. Peterson S. W. ( 2008 ). Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. . Mycologia 100, 205226. [View Article] [PubMed]
    [Google Scholar]
  12. Peterson S. W. ( 2012 ). Aspergillus and Penicillium identification using DNA sequences: barcode or MLST?. Appl Microbiol Biotechnol 95, 339344. [View Article] [PubMed]
    [Google Scholar]
  13. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  14. Samson R. A., Hong S., Peterson S. W., Frisvad J. C., Varga J. ( 2007 ). Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya . . Stud Mycol 59, 147203. [View Article] [PubMed]
    [Google Scholar]
  15. Samson R. A., Houbraken J., Thrane U., Frisvad J. C., Andersen B. ( 2010 ). Food and Indoor Fungi. Utrecht:: CBS KNAW Biodiversity Center;.
    [Google Scholar]
  16. Schoch C. L., Seifert K. A., Huhndorf S., Robert V., Spouge J. L., Levesque C. A., Chen W., Chen W. Fungal Barcoding Consortium ( 2012 ). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. . Proc Natl Acad Sci U S A 109, 62416246. [View Article] [PubMed]
    [Google Scholar]
  17. Sugui J. A., Vinh D. C., Nardone G., Shea Y. R., Chang Y. C., Zelazny A. M., Marr K. A., Holland S. M., Kwon-Chung K. J. ( 2010 ). Neosartorya udagawae (Aspergillus udagawae), an emerging agent of aspergillosis: how different is it from Aspergillus fumigatus?. J Clin Microbiol 48, 220228. [View Article] [PubMed]
    [Google Scholar]
  18. Takada M., Horie Y., Abliz P. ( 2001 ). Two new heterothallic Neosartorya from African soil. . Mycoscience 42, 361367. [View Article]
    [Google Scholar]
  19. Talavera G., Castresana J. ( 2007 ). Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. . Syst Biol 56, 564577. [View Article] [PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
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